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Immunofluorescent analysis of NEU2 in A549 cells using a NEU2 polyclonal antibody (Product # PA5-35114) followed by detection using a fluorescent conjugated secondary antibody (green). Nuclei were stained with Dapi (blue) and cytoplasmic actin was stained with a fluorescent phalloidin (red).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 23-50 amino acids from the N-terminal region of human NEU2|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
cytosolic sialidase; N-acetyl-alpha-neuraminidase 2; NEU2; neuraminidase 2 (cytosolic sialidase); sialidase 2 (cytosolic sialidase)