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Invitrogen
NF-H Monoclonal Antibody (RNF 405)
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FIGURE: 1 / 3
NF-H Antibody (OMA1-06117) in ICC/IF
JOR spine 2020 -
FIGURE 7 "Sholl" analysis of DRG neurons cultured at 2% and 20% oxygen for 2 days. A,B, Pattern images of DRG neurons (immunofluorescent staining with NF-200 antibody). C, Schematic showing the "Sholl" analysis of the outgrowth pattern. Circled samplings intersect with the neurite arbors providing information on how far the outgrowth can reach (represented as ending radius) and the number of branches (repre... View More
Product Details
OMA1-06117
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Sheep,
Rabbit,
Rat,
Xenopus,
Zebrafish
Published species
Bovine,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
RNF 405
Immunogen
Neurofilament preparation of calf brain tissue
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
OMA1-06117 reacts exclusively with the phosphorylated form of the 200 kDa neurofilament from human, mouse, rat, canine, rabbit, monkey, bovine, ovine, hamster, chicken, guinea pig, and Xenopus laevis tissues.
OMA1-06117 has been successfully used in Western blot and IHC (P) procedures. By Western blot, this antibody detects a single ~200 kDa protein representing phosphorylated neurofilament from rat brain homogenate.
OMA1-06117 antigen is cytoskeletal preparation of calf brain tissue.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 200 kDa neurofilament protein; heavy neurofilament protein; hSTEp; intermediate filament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide; neurofilament, heavy polypeptide 200kDa; neurofilament, heavy polypeptide L homeolog; neurofilament-H; NF 200 kD; NF-H; NHC; unnamed protein product
Gene Aliases: CMT2CC; I79_012512; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH; XELAEV_18007418mg
UniProt ID: (Human) P12036, (Mouse) P19246
structural constituent of cytoskeleton
protein binding
microtubule binding
kinesin binding
protein kinase binding
protein binding, bridging
dynein complex binding
structural constituent of postsynaptic intermediate filament cytoskeleton
structural molecule activity
toxic substance binding
nutrient reservoir activity
protein heterodimerization activity
microtubule cytoskeleton organization
axonogenesis
cell projection assembly
neurofilament bundle assembly
intermediate filament cytoskeleton organization
intermediate filament bundle assembly
peripheral nervous system neuron axonogenesis
neurofilament cytoskeleton organization
axon development
postsynaptic modulation of chemical synaptic transmission
postsynaptic intermediate filament cytoskeleton organization
regulation of organelle transport along microtubule
cellular response to leukemia inhibitory factor
ovarian follicle atresia
brain development
embryo development
spinal cord development
axon regeneration
neuron projection development
cellular response to oxidative stress
response to cocaine
cellular response to estradiol stimulus
response to sodium arsenite
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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