Bethyl Laboratories

NF1 Polyclonal Antibody

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NF1 Antibody in Immunoprecipitation (IP) — Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody A304-931A (lot A304-931A-1) used for IP at 6 µg per reaction. NF1 was also immunoprecipitated by rabbit anti-NF1 antibodies A304-932A and A300-140A. For blotting immunoprecipitated NF1, A304-931A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Product Details

Tested Applications

Dilution

Immunoprecipitation (IP)

2-10 µg/mg lysate

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Between 1 and 50

Conjugate

Unconjugated

Form

liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Mouse and Rat

Target Information

Neurofibromin is a member of the GTPase-activating (GAP) class of proteins. These proteins enhance the GTPase activity of signaling G-Proteins like Ras. The NF1 gene is also classified as a tumor suppressor gene, and many studies have shown that mutations at the NF1 locus produce neurofibromas, rhabdomyosarcomas, leimyosarcomas, mental retardation and memory deficiency. Neurofibromin has also been associated with the intermediated filaments of the cytoskeleton. NF1 is expressed at its highest levels during periods of desmosome formation and mutations of NF1 produce variable cell sizes and morphologies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Neurofibromatosis-related protein NF-1; Neurofibromin; Neurofibromin truncated; NF1; NFNS; VRNF; WSS

Gene Aliases: NF1; NFNS; VRNF; WSS

UniProt ID: (Human) P21359

Entrez Gene ID: (Human) 4763

Molecular Function: G-protein modulator enzyme modulator

MAPK cascade osteoblast differentiation metanephros development response to hypoxia liver development negative regulation of endothelial cell proliferation positive regulation of endothelial cell proliferation regulation of cell-matrix adhesion negative regulation of cell-matrix adhesion negative regulation of protein kinase activity cell communication Ras protein signal transduction negative regulation of neuroblast proliferation brain development peripheral nervous system development heart development skeletal muscle tissue development visual learning extrinsic apoptotic signaling pathway via death domain receptors regulation of gene expression Schwann cell development phosphatidylinositol 3-kinase signaling negative regulation of angiogenesis spinal cord development amygdala development forebrain astrocyte development neural tube development cerebral cortex development myelination in peripheral nervous system actin cytoskeleton organization extracellular matrix organization collagen fibril organization adrenal gland development negative regulation of cell migration regulation of synaptic transmission, GABAergic cellular response to heat negative regulation of Rac protein signal transduction wound healing negative regulation of transcription factor import into nucleus positive regulation of apoptotic process regulation of GTPase activity negative regulation of MAP kinase activity negative regulation of MAPK cascade pigmentation positive regulation of neuron apoptotic process regulation of blood vessel endothelial cell migration positive regulation of GTPase activity regulation of bone resorption negative regulation of osteoclast differentiation regulation of glial cell differentiation positive regulation of adenylate cyclase activity regulation of angiogenesis negative regulation of Ras protein signal transduction negative regulation of neurotransmitter secretion negative regulation of fibroblast proliferation regulation of long-term neuronal synaptic plasticity sympathetic nervous system development camera-type eye morphogenesis negative regulation of astrocyte differentiation negative regulation of oligodendrocyte differentiation smooth muscle tissue development artery morphogenesis forebrain morphogenesis cognition gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission observational learning regulation of long-term synaptic potentiation positive regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of extrinsic apoptotic signaling pathway in absence of ligand

Process(es)

GTPase activator activity protein binding phosphatidylethanolamine binding phosphatidylcholine binding