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Invitrogen
NF2 Polyclonal Antibody
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FIGURE: 1 / 3
NF2 Antibody (PA5-89329) in WB
Western blot analysis of NF2 in extracts from normal (control) and NF2 Rabbit pAb knockout (KO) HeLa cells. Samples were incubated with NF2 Polyclonal antibody (Product # PA5-89329) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-89329
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 477-576 of human NF2 (NP_0002591)
Conjugate
Form
Liquid
Concentration
3.36 mg/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: A549, A431, HeLa, SKOV3, Mouse brain, Mouse kidney, Mouse heart, Rat brain; Cellular Location: Cell projection, Cytoplasm, Cytoplasmic granule, Cytoplasmic side, Nucleus, Nucleus, Peripheral membrane protein, cytoskeleton, filopodium membrane, perinuclear region, ruffle membrane
Immunogen sequence: TKPTYPPMNP IPAPLPPDIP SFNLIGDSLS FDFKDTDMKR LSMEIEKEKV EYMEKSKHLQ EQLNELKTEI EALKLKERET ALDILHNENS DRGGSSKHNT
Target Information
NF2 encodes a protein, Merlin, that is similar to some members of the ERM family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. Merlin has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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