Search
Invitrogen
NFATC3 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 6
NFATC3 Antibody (PA5-79734) in WB
Western blot analysis of NFATC3 in Lane 1: human 22RV1 whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with NFATC3 polyclonal antibody (Product # PA5-79734) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP second... View More
Product Details
PA5-79734
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human NFAT4 recombinant protein (Position: K630-L712).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
4mg trehalose, 0.9mg NaCl, 0.2mg Na2PO4
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human 22RV1 whole cell. Flow: A431 cell, U20S cell, K562 cell, SiHa cell.
Target Information
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alternative splicing form; NF-AT4; NF-ATc3; NFAC3; NFATc3; NFATx; NFC3; Nuclear factor of activated T-cells, cytoplasmic 3; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3; T cell transcription factor NFAT4; T-cell transcription factor NFAT4; transcription factor; unnamed protein product
Gene Aliases: C80703; D8Ertd281e; n339260; NF-AT4c; NFAT4; NFATC3; NFATX
UniProt ID: (Human) Q12968, (Rat) A0A0G2JTY4
Entrez Gene ID: (Human) 4775, (Rat) 361400, (Mouse) 18021
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific double-stranded DNA binding
core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
chromatin binding
transcription factor binding
sequence-specific DNA binding
Rel homology transcription factor
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
inflammatory response
calcineurin-NFAT signaling cascade
positive thymic T cell selection
positive regulation of nitric oxide biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
negative regulation of pri-miRNA transcription from RNA polymerase II promoter
DN4 thymocyte differentiation
negative regulation of vascular smooth muscle cell differentiation
positive regulation of artery morphogenesis
patterning of blood vessels
response to hypoxia
blood vessel remodeling
heart development
cardiac muscle hypertrophy in response to stress
myotube differentiation
myotube cell development
T cell differentiation
cellular respiration
positive regulation of transcription, DNA-templated
thymus development
skeletal muscle fiber development
smooth muscle cell differentiation
muscle cell development
cellular response to calcium ion
cellular response to lithium ion
regulation of store-operated calcium entry
transcription, DNA-templated
transcription from RNA polymerase II promoter
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support