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Invitrogen
NFIA Polyclonal Antibody
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Product Details
PA5-79735
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NFIA (180-224aa AYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSG VFS).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: JURKAT whole cell, COLO320 whole cell, mouse HEPA1-6 whole cell, mouse SP2/0 whole cell. IHC: mouse liver tissue, rat liver tissue, human mammary cancer tissue. Flow: K562 cell, SiHa cell, U20S cell.
Target Information
NF-1, also designated CTF, consists of a family of CCAAT box binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA binding domain, and activates transcription through a putatively novel, proline-rich, carboxy terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, alpha-globin, HSP 70, GRP 78, Histone H1, myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CCAAT-box-binding transcription factor; CTF; NF-I/A; NF1-A; NFI-A; Nuclear factor 1 A-type; nuclear factor 1/A; Nuclear factor I/A; TGGCA-binding protein; unnamed protein product
Gene Aliases: 1110047K16Rik; 9430022M17Rik; BRMUTD; C1DELp32p31; CTF; DEL1P32P31; KIAA1439; NF-I/A; NF1-A; NF1A; Nf1l21; NFI-A; NFI-L; NFIA
UniProt ID: (Human) Q12857, (Rat) P09414, (Mouse) Q02780
Entrez Gene ID: (Human) 4774, (Rat) 25492, (Mouse) 18027
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
DNA-binding transcription factor binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor binding
DNA-binding transcription factor
gene-specific transcriptional regulator
cell morphogenesis
DNA replication
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
response to wounding
exit from mitosis
gene expression
glial cell proliferation
viral genome replication
glial cell fate specification
neurogenesis
BMP signaling pathway
limb morphogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
neuron fate specification
generation of neurons
cartilage development
retina development in camera-type eye
synapse maturation
neural precursor cell proliferation
ureter development
negative regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
response to organic cyclic compound
transcription, DNA-templated
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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