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Invitrogen
NFIL3 Polyclonal Antibody
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FIGURE: 1 / 3
NFIL3 Antibody (PA5-106504) in ICC/IF
Immunocytochemistry analysis of NFIL3 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NFIL3 polyclonal antibody (Product # PA5-106504) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106504
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NFIL3(Accession Q16649), corresponding to amino acid residues E429-G462.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NFIL3.
Target Information
Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adenovirus E4 promoter region binding protein; E4 promoter binding-protein 4; E4 promoter-binding protein 4; E4BP4; Embryo implantation-related NFIL3/E4BP4-like transcription factor; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; NF IL3A; NFIL3; Nuclear factor interleukin-3-regulated protein; OTTHUMP00000021633; Transcriptional activator NF-IL3A; unnamed protein product
Gene Aliases: AV225605; E4BP4; IL3BP1; NF-IL3A; NFIL3; NFIL3A
UniProt ID: (Human) Q16649, (Mouse) O08750, (Rat) Q6IMZ0
Entrez Gene ID: (Human) 4783, (Mouse) 18030, (Rat) 114519
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
identical protein binding
RNA polymerase II core promoter sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
sequence-specific DNA binding
basic leucine zipper transcription factor
DNA-binding transcription factor
gene-specific transcriptional regulator
negative regulation of transcription from RNA polymerase II promoter
natural killer cell differentiation
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
immune response
circadian rhythm
positive regulation of gene expression
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
rhythmic process
cellular response to interleukin-4
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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