Western blot analysis of NFKBIL2 using A) 30 µg H1299 whole cell lysate (B) 30 µg HCT116 whole cell lysate and C) 30 µg MCF-7 whole cell lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a NFKBIL2 polyclonal antibody (Product # PA5-31338) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 130 and 410 of Human NFKBIL2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31338 targets NFKBIL2 in WB applications and shows reactivity with Human samples.
The PA5-31338 immunogen is recombinant fragment corresponding to a region within amino acids 130 and 410 of Human NFKBIL2.
The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus.
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