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Invitrogen
NFYA Polyclonal Antibody
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FIGURE: 1 / 7
NFYA Antibody (PA5-118825) in IHC (P)
Immunohistochemical analysis of NFYA in Human colorectal cancer and adjacent normal tissues using a NFYA polyclonal antibody (Product # PA5-118825). The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary ... View More
Product Details
PA5-118825
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NFYA(Accession P23511), corresponding to amino acid residues K270-S320.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NFYA.
Target Information
The NFYA gene encodes for one of the subunits of the Nuclear Factor Y (NF-Y) transcription factor, which plays a critical role in the regulation of various genes, particularly those containing the CCAAT box within their promoter regions. Structurally, NF-Y consists of a heterotrimeric complex made up of NF-YA, NF-YB, and NF-YC subunits. NF-YA serves as the sequence-specific DNA-binding component, whereas the NF-YB/NF-YC heterodimer forms a Histone Fold Domain crucial for DNA binding and stabilization. Functionally, NF-YA is pivotal in cell cycle regulation and neuronal development, influencing neural progenitor maintenance during brain morphogenesis. It has been implicated in various physiological processes, including response to plant stress and cancer progression. Specifically, NFYA gene activity is prevalent in proliferating cells and is often dysregulated in cancerous tissues, which highlights its potential as a therapeutic target. NFYA's expression in plant systems, such as in the Arabidopsis species, is modulated by environmental stresses like drought, indicating its broader impact on biological stress responses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CAAT box DNA-binding protein subunit A; CAAT-box DNA binding protein subunit A; CAAT-box DNA-binding protein subunit A; CBF-A; CBF-B; CCAAT-binding transcription factor subunit A; CCAAT-binding transcription factor subunit B; FLJ11236; HAP2 CCAAT-binding protein; nf-y; NF-YA; Nuclear transcription factor Y subunit A; Nuclear transcription factor Y subunit alpha; nuclear transcription factor Y, alpha; Transcription factor NF-Y, A subunit
Gene Aliases: AA407810; CBF-A; CBF-B; HAP2; NF-YA; NFYA; SEZ-10; SEZ10
UniProt ID: (Human) P23511, (Mouse) P23708, (Rat) P18576
Entrez Gene ID: (Human) 4800, (Mouse) 18044, (Rat) 29508
core promoter sequence-specific DNA binding
transcription cofactor binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription regulatory region DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor binding
DNA-binding transcription factor
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
positive regulation of transcription, DNA-templated
rhythmic process
transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
regulation of stem cell population maintenance
positive regulation of stem cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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