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Invitrogen
NFYC Polyclonal Antibody
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FIGURE: 1 / 1
NFYC Antibody (PA5-117162) in WB
Western blot analysis of NFYC in rat thymus. Samples were incubated with NFYC Polyclonal antibody (Product # PA5-117162) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-117162
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Amount
80 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse kidney, Mouse heart
Immunogen sequence: MEEIRNLTVK DFRVQELPLA RIKKIMKLDE DVKMISAEAP VLFAKAAQIF ITELTLRAWI HTEDNKRRTL QRNDIAMAIT KFDQFDFLID IVPR
Target Information
The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein histone-fold "handshake" motif in a manner analogous to the histone proteins, H2B and H2A, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CAAT box DNA-binding protein subunit C; DKFZp667G242; FLJ45775; NF-YC; Nuclear transcription factor Y subunit C; Nuclear transcription factor Y subunit gamma
Gene Aliases: Nfyc
UniProt ID: (Mouse) P70353
Entrez Gene ID: (Mouse) 18046
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
DNA-binding transcription factor
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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