Immunofluorescence analysis of NFkB p105 / p50 Antibody was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with NFkB p105 / p50 Antibody (385600) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing Nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Virus, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein derived from an internal region of the human NF-kappa-B (p50) (nuclear factor of kappa-B).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Gel Shift (GS)||5-10 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
NF-kB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. It is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50), a 50KDa functional sub-unit of NF-kB, is a member of Rel protein family. It is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NF-kB factor. NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-bidning. Pathological role of NF-kB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.
IP-MS enrichment of NFKB1 (LFQ intensity): NFKB1 was enriched 342-fold from LNCAP lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and NFKB1 antibody (Part No. 51-3500). See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Superpulsed (Ga-As, 904 nm) low-level laser therapy (LLLT) attenuates inflammatory response and enhances healing of burn wounds.
51-3500 was used in western blot to examine the effects of superpulsed laser on the healing of burn wounds in rats and investigate the associated mechanisms of action.
|Gupta A,Keshri GK,Yadav A,Gola S,Chauhan S,Salhan AK,Bala Singh S||Journal of biophotonics (8:489)||2015|
Human gut flora-fermented nondigestible fraction from cooked bean ( Phaseolus vulgaris L.) modifies protein expression associated with apoptosis, cell cycle arrest, and proliferation in human adenocarcinoma colon cancer cells.
51-3500 was used in western blot to assess the effects of fermentation products produced by the human gut flora on human adenocarcinoma colon cancer cells.
|Campos-Vega R,García-Gasca T,Guevara-Gonzalez R,Ramos-Gomez M,Oomah BD,Loarca-Piña G||Journal of agricultural and food chemistry (60:12443)||2012|
|Measles virus-induced modulation of host-cell gene expression.||Bolt G,Berg K,Blixenkrone-Møller M||The Journal of general virology (83:1157)||2002|
|Mediation of proliferating cell nuclear antigen (PCNA)-dependent DNA replication through a conserved p21(Cip1)-like PCNA-binding motif present in the third subunit of human DNA polymerase delta.||Ducoux M,Urbach S,Baldacci G,Hübscher U,Koundrioukoff S,Christensen J,Hughes P||The Journal of biological chemistry (276:49258)||2001|