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Invitrogen
NG2 Polyclonal Antibody
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FIGURE: 1 / 3
NG2 Antibody (PA5-92029) in WB
Western Blot analysis of NG2 in extracts from normal (control) and CSPG4 knockout (KO) HeLa cells using NG2 Polyclonal Antibody (Product # PA5-92029) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Product Details
PA5-92029
Applications
Tested Dilution
Publications
Product Specifications
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2.
Conjugate
Form
Liquid
Concentration
0.44 mg/mL
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
Immunogen sequence: LGAPLEGVLV ELEVLPAAIP LEAQNFSVPE GGSLTLAPPL LRVSGPYFPT LLGLSLQVLE PPQHGALQKE DGPQARTLSA FSWRMVEEQL IRYVHDGSET LTDSFVLMAN ASEMDRQSHP VAFTVTVLPV NDQPPILTTN TGLQMWEGAT APIPAEALRS TDGDSGSEDL VYTIEQPSNG RVVLRGAPGT EVRSFTQAQL DGGLVLFSHR GTLDGGFRFR LSDG
Positive Samples: A-375, HeLa; Cellular Location: Apical cell membrane, Cell projection, Extracellular side, Single-pass type I membrane protein, lamellipodium membrane
Target Information
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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