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FIGURE: 1 / 6
NGAL Antibody (711280) in ICC/IF
For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous Lipocalin2 using Anti-Lipocalin2 Recombinant Rabbit Superclonal™ Antibody (Product # 711280, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) is stained using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938) and cytoskeletal F-actin (red) staining using Rhodamine Phalloidin (Product # R415, 1:300) Panel a-d) shows r... View More
Product Details
711280
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Immunogen
Protein corresponding to human NGAL (aa21-aa198)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Rabbit, Sheep.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HGNC:6526; LCN2; Lipocalin-2; Lipocalin2; migration-stimulating factor inhibitor; Neutrophil gelatinase-associated lipocalin; neutrophil lipocalin; NGAL; Oncogene 24p3; OTTHUMP00000022240; p25; RP23-161B9.11; Siderocalin; siderocalin LCN2; unnamed protein product; uterocalin
Gene Aliases: 24p3; HNL; LCN2; MSFI; NGAL; p25
UniProt ID: (Human) P80188
Entrez Gene ID: (Human) 3934
immune system process
ion transport
iron ion transport
apoptotic process
acute-phase response
response to oxidative stress
short-term memory
long-term memory
response to xenobiotic stimulus
response to virus
response to bacterium
response to herbicide
response to toxic substance
response to blue light
response to fructose
response to iron(II) ion
response to mycotoxin
positive regulation of endothelial cell migration
positive regulation of gene expression
siderophore transport
positive regulation of cell projection organization
response to nutrient levels
cellular response to nutrient levels
response to lipopolysaccharide
cellular response to increased oxygen levels
defense response to bacterium
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
innate immune response
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to interleukin-1
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to hypoxia
cellular response to X-ray
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of hippocampal neuron apoptotic process
negative regulation of hippocampal neuron apoptotic process
positive regulation of cold-induced thermogenesis
positive regulation of reactive oxygen species biosynthetic process
response to kainic acid
positive regulation of iron ion import across plasma membrane
cellular response to beta-amyloid
positive regulation of endothelial tube morphogenesis
cellular response to nerve growth factor stimulus
positive regulation of reactive oxygen species metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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