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NGEF Antibody (PA5-52521) in IHC
Cell death & disease 2021 -
Fig. 1 Ephexin1 is upregulated in CRC and LC and promotes tumor cell growth and proliferation. A Expression of Ephexin1 mRNA in 12 primary cancer types from TCGA. Data are shown as mean +- SD. ns not significant, *** P < 0.001, two-tailed Student's t test. B Kaplan-Meier analysis of overall survival according to Ephexin1 in CRC and LC patients. P values are for a log-rank test. C Immunohistochemistry (IHC) ... View More
Product Details
PA5-52521
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human NGEF
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2644657
Product Specific Information
Immunogen sequence: METRESEDLE KTRRKSASDQ WNTDNEPAKV KPELLPEKEE TSQADQDIQD KEPHCHIPIK RNSIFNRSIR RKSKAKARDN PERNASCLAD SQDNGKSVNE PLTLNIPWSR TPPCRTAMQT DPGAQEMSES SST
Highest antigen sequence identity to the following orthologs: Mouse - 48%, Rat - 26%.
Target Information
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Eph-interacting exchange protein; Ephexin-1; Neuronal guanine nucleotide exchange factor
Gene Aliases: ARHGEF27; EPHEXIN; NGEF
UniProt ID: (Human) Q8N5V2
Entrez Gene ID: (Human) 25791
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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