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Invitrogen
NGF Polyclonal Antibody
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FIGURE: 1 / 2
NGF Antibody (OSN00002A) in WB
Western blot was performed using Anti-NGF Polyclonal Antibody (Product # OSN00002A) and a 30 kDa band corresponding to NGF was observed across in Mouse Salivary Gland and not in Mouse Brain and Rat Salivary Gland. Tissue extracts (30 µg lysate) of Mouse Salivary gland (Lane 1), Mouse Brain (Lane 2), Rat Salivary gland (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product #... View More
Product Details
OSN00002A
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Native beta-nerve growth factor
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Tested in vitro, this antibody abolishes the neuronal survival and the outgrowth actions of murine NGF in chicken DRG.
Reconstitute in 100 µL sterile water. Centrifuge to remove insoluble material. Use at a concentration of 1-5 µg/mL.
Target Information
Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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