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Invitrogen
NGF Polyclonal Antibody
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FIGURE: 1 / 1
NGF Antibody (PA1-18378) in Neu
Nature communications 2018 -
Fig. 6 mEERL exosomes induce neurite outgrowth without NGF. a PC12 cells were stimulated with 100 ng/mL of recombinant NGF (NGF), mEERL parental (parental) or mEERL EphrinB1 (EphrinB1) conditioned media with or without neutralizing anti-NGF antibody (+Ab); 24 h later, cells were fixed and stained for beta-III tubulin expression which was then quantified. Statistical analysis by two-way ANOVA with post h... View More
Product Details
PA1-18378
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Native mouse beta NGF purified from submaxillary salivary gland.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Wet ice
RRID
Product Specific Information
By ELISA, this antibody exhibits a cross-reactivity of less than 1% to recombinant human BDNF, NT3 and NT4/5. It will not react with bovine NGF.
Use at 1-3 µg/mL for inhibition of biological activity in vitro. Use neat for in vivo studies at 2-10 µg/mL (ED50). This antiserum completely inhibits neuronal survival and the outgrowth actions of murine NGF in chicken DRG in vitro.
Reconstitute with 20 µL of distilled water. After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent.
Target Information
Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. NGF exhibits nerve growth stimulating activity and is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Further, NGF stimulates division and differentiation of sympathetic and embryonic sensory neurons. Mutations in the NGF gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF gene expression is associated with allergic rhinitis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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