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Zeta
NGFR Monoclonal Antibody (ZM55), MonoMab™
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Product Details
Z2365MS
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
ZM55
Immunogen
Recombinant human p75 NGFR protein fragment (around aa 281-421)
Conjugate
Form
Liquid
Concentration
100 µg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
tris with NP-40, BSA
Contains
<0.1% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
A recommended positive control tissue for this product is reast sclerosing adenosis, however positive controls are not limited to this tissue type.
The primary antibody is intended for laboratory professional use in the detection of the corresponding protein in formalin-fixed, paraffin-embedded tissue stained in manual qualitative immunohistochemistry (IHC) testing. This antibody is intended to be used after the primary diagnosis of tumor has been made by conventional histopathology using non-immunological histochemical stains.
Nerve growth factor receptor (p75NGFR), is the first of neurotrophin receptors to be isolated and is a member of the tumor necrosis factor (TNF) receptor family. NGFR has been shown to be a reliable marker for desmoplastic and neurotropic melanoma by several groups. It is now apparent that expression of NGFR is ubiquitous and not limited to the nervous system, being expressed in mature nonneural cells such as perivascular cells, follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells and myoepithelial cells. Studies in prostate and urothelial cancer suggest that NGFR may act as a tumor suppressor, negatively regulating cell growth and proliferation. Anti-NGFR labels the myoepithelial cells of breast ducts and intralobular fibroblasts of breast ducts and thus aides in the diagnosis of malignancy in the breast.
Antibody is used with formalin-fixed and paraffin-embedded sections. Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended. In general, immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody (link antibody), an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
A positive tissue control must be run with every staining procedure performed. This tissue may contain both positive and negative staining cells or tissue components and serve as both the positive and negative control tissue. External Positive control materials should be fresh autopsy/biopsy/surgical specimens fixed, processed and embedded as soon as possible in the same manner as the patient sample (s). Positive tissue controls are indicative of correctly prepared tissues and proper staining methods. The tissues used for the external positive control materials should be selected from the patient specimens with well-characterized low levels of the positive target activity that gives weak positive staining. The low level of positivity for external positive controls is designed to ensure detection of subtle changes in the primary antibody sensitivity from instability or problems with the staining methodology. A tissue with weak positive staining is more suitable for optimal quality control and for detecting minor levels of reagent degradation.
Internal or external negative control tissue may be used depending on the guidelines and policies that govern the organization to which the end user belongs to. The variety of cell types present in many tissue sections offers internal negative control sites, but this should be verified by the user. The components that do not stain should demonstrate the absence of specific staining, and provide an indication of non-specific background staining. If specific staining occurs in the negative tissue control sites, results with the patient specimens must be considered invalid.
Target Information
Neurotrophic factors control the survival, differentiation and maintenance of neurons in the peripheral and central nervous systems, and of other neural crest-derived cell types. Developing sympathetic neurons are absolutely dependent upon nerve growth factor (NGF) during the period of target competition in vivo. Antibodies reacting specifically with NGFR p75 are useful tools in the detection and characterization of NGFR p75, to enhance our understanding of a wide range of phenomena in the development, plasticity and repair of the nervous system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD271; CD271 antigen; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; Low-affinity nerve growth factor receptor p75NGFR; Low-affinity nerve growth factor receptor p75NGR; NGF receptor; p-75; p75 ICD; p75 ntr; TNFR superfamily, member 16; TNR16; Tumor necrosis factor receptor superfamily member 16; unnamed protein product
Gene Aliases: CD271; Gp80-LNGFR; NGFR; p75(NTR); p75NTR; TNFRSF16
UniProt ID: (Human) P08138
Entrez Gene ID: (Human) 4804
cellular glucose homeostasis
hair follicle development
intracellular protein transport
apoptotic process
signal transduction
Rho protein signal transduction
nervous system development
axon guidance
central nervous system development
circadian rhythm
fibroblast growth factor receptor signaling pathway
regulation of gene expression
detection of temperature stimulus
nerve development
cell differentiation
negative regulation of cell migration
hair follicle morphogenesis
circadian regulation of gene expression
dorsal aorta development
negative regulation of fibroblast growth factor receptor signaling pathway
regulation of cell proliferation
odontogenesis of dentin-containing tooth
positive regulation of odontogenesis of dentin-containing tooth
glucose homeostasis
positive regulation of apoptotic process
skin development
fibroblast proliferation
positive regulation of fibroblast proliferation
rhythmic process
neuron apoptotic process
negative regulation of hair follicle development
extrinsic apoptotic signaling pathway
presynaptic modulation of chemical synaptic transmission
positive regulation of protein localization to nucleus
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis
cellular response to beta-amyloid
positive regulation of endothelial cell apoptotic process
positive regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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