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Product Details
NHERF1-101AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids within region 255-305 on human Na(+)/H(+) exchange regulatory cofactor NHE-RF1.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5-1.5 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
proprietary buffer with 30% glycerol
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EBP50; ERM-binding phosphoprotein; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
Gene Aliases: EBP-50; EBP50; NHE-RF; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1
UniProt ID: (Human) O14745, (Rat) Q9JJ19, (Mouse) P70441
Entrez Gene ID: (Human) 9368, (Rat) 59114, (Mouse) 26941
receptor binding
protein binding
beta-catenin binding
chloride channel regulator activity
phosphatase binding
PDZ domain binding
beta-2 adrenergic receptor binding
protein complex binding
protein complex scaffold
protein self-association
dopamine receptor binding
growth factor receptor binding
protein domain specific binding
renal sodium ion transport
adenylate cyclase-activating dopamine receptor signaling pathway
sensory perception of sound
negative regulation of cell proliferation
negative regulation of platelet-derived growth factor receptor signaling pathway
negative regulation of sodium ion transport
negative regulation of phosphatidylinositol 3-kinase signaling
Wnt signaling pathway
cAMP-mediated signaling
microvillus assembly
actin cytoskeleton organization
negative regulation of cell migration
cellular phosphate ion homeostasis
negative regulation of sodium:proton antiporter activity
bile acid secretion
cellular protein localization
glutathione transport
regulation of excretion
regulation of protein kinase activity
negative regulation of protein kinase B signaling
auditory receptor cell stereocilium organization
phospholipase C-activating dopamine receptor signaling pathway
renal absorption
negative regulation of ERK1 and ERK2 cascade
renal phosphate ion absorption
negative regulation of cell motility
positive regulation of intrinsic apoptotic signaling pathway
protein complex assembly
regulation of cell shape
regulation of cell size
gland morphogenesis
regulation of sodium:proton antiporter activity
negative regulation of catenin import into nucleus
establishment of nucleus localization
establishment of epithelial cell apical/basal polarity
negative regulation of mitotic cell cycle
establishment of Golgi localization
establishment of protein localization to plasma membrane
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