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Product Details
A302-975A
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:2,000-1:10,000 | - |
Immunohistochemistry (IHC) |
1:100-1:500 | - |
Immunoprecipitation (IP) |
2-5 µg/mg lysate | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Between 325 and 358 |
Conjugate |
Unconjugated |
Form |
liquid |
Concentration |
0.20 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
TBS, pH 7.0 to 8.0, with 0.1% BSA |
Contains |
0.09% sodium azide |
Storage conditions |
4° C |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat, Chicken and Bovine
Target Information
NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EBP50; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
Gene Aliases: EBP50; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1
UniProt ID: (Human) O14745
Entrez Gene ID: (Human) 9368
renal sodium ion transport
protein complex assembly
adenylate cyclase-activating dopamine receptor signaling pathway
sensory perception of sound
negative regulation of cell proliferation
regulation of cell shape
regulation of cell size
negative regulation of platelet-derived growth factor receptor signaling pathway
negative regulation of phosphatidylinositol 3-kinase signaling
Wnt signaling pathway
cAMP-mediated signaling
gland morphogenesis
microvillus assembly
actin cytoskeleton organization
negative regulation of cell migration
cellular phosphate ion homeostasis
regulation of sodium:proton antiporter activity
negative regulation of sodium:proton antiporter activity
bile acid secretion
glutathione transport
negative regulation of catenin import into nucleus
establishment of nucleus localization
regulation of excretion
establishment of epithelial cell apical/basal polarity
regulation of protein kinase activity
negative regulation of mitotic cell cycle
establishment of Golgi localization
negative regulation of protein kinase B signaling
auditory receptor cell stereocilium organization
phospholipase C-activating dopamine receptor signaling pathway
renal absorption
negative regulation of ERK1 and ERK2 cascade
establishment of protein localization to plasma membrane
renal phosphate ion absorption
positive regulation of intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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