Proteintech

NHP2 Polyclonal Antibody

NHP2 Antibody in Immunocytochemistry (ICC/IF)

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NHP2 Antibody (15128-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using NHP2 antibody 15128-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

NHP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NHP2 Antibody in Immunoprecipitation (IP)

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Product Details

15128-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2400

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NHP2 Fusion Protein Ag7251 (1-153 aa encoded by BC000009)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTKIKADPD GPEAQAEACS GERTYQELLV NQNPIAQPLA SRRLTRKLYK CIKKAVKQKQ IRRGVKEVQK FVNKGEKGIM VLAGDTLPIE VYCHLPVMCE DRNLPYVYIP SKTDLGAAAG SKRPTCVIMV KPHEEYQEAY DECLEEVQSL PLPL (1-153 aa encoded by BC000009)

Target Information

NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Proteomic analyses reveal new features of the box H/ACA RNP biogenesis.

Nucleic acids research

Schlotter F,Mérouani S,Flayac J,Kogey V,Issa A,Dodré M,Huttin A,Branlant C,Bertrand E,Labialle S,Vandermoere F,Verheggen C,Massenet S

Mon Apr 24 00:00:00 UTC 2023

Proteomic analyses reveal new features of the box H/ACA RNP biogenesis.

Nucleic acids research

Schlotter F,Mérouani S,Flayac J,Kogey V,Issa A,Dodré M,Huttin A,Branlant C,Bertrand E,Labialle S,Vandermoere F,Verheggen C,Massenet S

Mon Apr 24 00:00:00 UTC 2023

Chromatin-associated orphan snoRNA regulates DNA damage-mediated differentiation via a non-canonical complex.

Cell reports

Han C,Sun LY,Luo XQ,Pan Q,Sun YM,Zeng ZC,Chen TQ,Huang W,Fang K,Wang WT,Chen YQ

Tue Mar 29 00:00:00 UTC 2022

Comparative Proteomic Analysis of tPVAT during Ang II Infusion.

Biomedicines

Liang X,Guan H,Sun J,Qi Y,Yao W

Thu Dec 02 00:00:00 UTC 2021

NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.

The EMBO journal

Raghunandan M,Geelen D,Majerova E,Decottignies A

Mon Mar 15 00:00:00 UTC 2021

NHP2 downregulation counteracts hTR-mediated activation of the DNA damage response at ALT telomeres.

The EMBO journal

Raghunandan M,Geelen D,Majerova E,Decottignies A

Mon Mar 15 00:00:00 UTC 2021

SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization.

Molecular and cellular biology

MacNeil DE,Lambert-Lanteigne P,Qin J,McManus FP,Bonneil E,Thibault P,Autexier C

Wed Mar 24 00:00:00 UTC 2021

Structure of human telomerase holoenzyme with bound telomeric DNA.

Nature

Ghanim GE,Fountain AJ,van Roon AM,Rangan R,Das R,Collins K,Nguyen THD

Sat May 01 00:00:00 UTC 2021

Nhp2 is a reader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis.

EMBO reports

Mawer JSP,Massen J,Reichert C,Grabenhorst N,Mylonas C,Tessarz P

Tue Oct 05 00:00:00 UTC 2021

N-terminal residues of human dyskerin are required for interactions with telomerase RNA that prevent RNA degradation.

Nucleic acids research

MacNeil DE,Lambert-Lanteigne P,Autexier C

Tue Jun 04 00:00:00 UTC 2019

Cryo-EM structure of substrate-bound human telomerase holoenzyme.

Nature

Nguyen THD,Tam J,Wu RA,Greber BJ,Toso D,Nogales E,Collins K

Tue May 01 00:00:00 UTC 2018

Cryo-EM structure of substrate-bound human telomerase holoenzyme.

Nature

Nguyen THD,Tam J,Wu RA,Greber BJ,Toso D,Nogales E,Collins K

Tue May 01 00:00:00 UTC 2018

Differential requirements for H/ACA ribonucleoprotein components in cell proliferation and response to DNA damage.

Histochemistry and cell biology

Lin P,Mobasher ME,Hakakian Y,Kakarla V,Naseem AF,Ziai H,Alawi F

Tue Dec 01 00:00:00 UTC 2015

Differential requirements for H/ACA ribonucleoprotein components in cell proliferation and response to DNA damage.

Histochemistry and cell biology

Lin P,Mobasher ME,Hakakian Y,Kakarla V,Naseem AF,Ziai H,Alawi F

Tue Dec 01 00:00:00 UTC 2015

Expressional analysis of NOLA1, NOLA2, NOLA3 and DKC1, the core proteins in H/ACA riboproteins, in gastric and colorectal cancers.

Pathology

Kim MS,Kim SS,Yoo NJ,Lee SH

Mon Oct 01 00:00:00 UTC 2012

Expressional analysis of NOLA1, NOLA2, NOLA3 and DKC1, the core proteins in H/ACA riboproteins, in gastric and colorectal cancers.

Pathology

Kim MS,Kim SS,Yoo NJ,Lee SH

Mon Oct 01 00:00:00 UTC 2012

A spectrum of oncocytic lesions in the parotid gland of a patient with sarcoidosis.

Pathology

Ziad F,Sinha S,White J,Pandya H

Mon Oct 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: H/ACA ribonucleoprotein complex subunit 2; NHP2 ribonucleoprotein homolog; NHP2-like protein; Nucleolar protein family A member 2; nucleolar protein family A, member 2 (H/ACA small nucleolar RNPs); snoRNP protein NHP2

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Gene Aliases: DKCB2; HSPC286; NHP2; NHP2P; NOLA2

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UniProt ID: (Human) Q9NX24

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Entrez Gene ID: (Human) 55651

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Function(s)

protein binding snoRNA binding box H/ACA snoRNA binding poly(A) RNA binding telomerase RNA binding ribosomal protein

Process(es)

cleavage involved in rRNA processing maturation of LSU-rRNA translation telomere maintenance via telomerase rRNA pseudouridine synthesis snRNA pseudouridine synthesis positive regulation of telomerase RNA localization to Cajal body

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NHP2 Polyclonal Antibody

NHP2 Antibody (15128-1-AP) in ICC/IF

Product Details

15128-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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