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|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 75-90 of mouse NIFK.|
|Storage buffer||50mM sodium borate|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for MKI67IP (transient overexpression lysate), NIH-3T3 whole cell lysate.
NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
hNIFK; Ki67ip; MKI67 (FHA domain) interacting nucleolar phosphoprotein; MKI67 FHA domain-interacting nucleolar phosphoprotein; mNIFK; nucleolar phosphoprotein Nopp34; nucleolar protein interacting with the FHA domain of pKI-67
AI852665; C130020J04Rik; MKI67IP; NIFK; NOPP34