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Invitrogen
NIK Polyclonal Antibody
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FIGURE: 1 / 1
NIK Antibody (PA5-88155) in WB
Western blot analysis of NIK in extracts of Rat lung. Samples were incubated with NIK Polyclonal antibody (Product # PA5-88155) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-88155
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Protein (or fragment)
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Amount
33 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: KFSVEEYLVH ALQGSVSSGQ AHSLTSLAKT WAARGSRSRE PSPKTEDNEG VLLTEKLKPV DYEYREEVHW ATHQLRLGRG SFGEVHRMED KQTGFQCAVK KVRLEVFRAE ELMACAGLTS PRIVPLYGAV REGPWVNIFM ELLEGGSLGQ LVKEQGCLPE DRALYYLGQA LEGLEYLHSR RILHGDVKAD NVLLSSDGSH AALCDFGHAV CLQPDGLGKS LLTGDYIPGT ETHMAPEVVL GRSCDAKVDV WSSCCMMLHM LNGCHPWTQF FRGPLCLKIA SEPPPVREIP PSCAPLTAQA IQEGLRKEPI HRVSAAELGG KVNRALQQVG GLK
Positive Samples: Raji; Cellular Location: Cytoplasm
Target Information
MAP3K14 (NIK) is structurally related to MAP3Ks. MAP3K14 phosphorylates and activates CHUK (IKKalpha) and IKBKB (IKK beta), which in turn phosphorylate IkB-alpha, releasing NF-KappaB leading to up-regulation of inflammatory response genes. This kinase binds to TRAF2 and stimulates NF-kappaB activity. It shares sequence similarity with several other MAPKK kinases. It participates in an NF-kappaB-inducing signalling cascade common to receptors of the tumour-necrosis/nerve-growth factor (TNF/NGF) family and to the interleukin-1 type-I receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: mitogen-activated protein kinase kinase kinase 14
Entrez Gene ID: (Rat) 360640
MAPK cascade
activation of MAPK activity
protein phosphorylation
immune response
I-kappaB kinase/NF-kappaB signaling
signal transduction by protein phosphorylation
intracellular signal transduction
NIK/NF-kappaB signaling
positive regulation of I-kappaB kinase/NF-kappaB signaling
cellular response to mechanical stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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