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Bethyl Laboratories
NISCH Polyclonal Antibody
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FIGURE: 1 / 1
NISCH Antibody (A305-446A) in IP
Detection of human NISCH by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NISCH antibody (Product # A305-446A) (lot 1) used for IP at 6 µg per reaction. NISCH was also immunoprecipitated by rabbit anti-NISCH antibody BL21611. For blotting immunoprecipitated NISCH, A305-446A was used at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-446A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 1100 to 1150 of human Nischarin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension. Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons. Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hIRAS; I-1; I-1 receptor candidate protein; I1R; I1R candidate protein; Imidazoline receptor 1; imidazoline receptor antisera selected; Imidazoline receptor antisera-selected protein; Imidazoline-1 receptor; Imidazoline-1 receptor candidate protein; Nischarin
Gene Aliases: hIRAS; I-1; IR1; IRAS; KIAA0975; NISCH
UniProt ID: (Human) Q9Y2I1
Entrez Gene ID: (Human) 11188
Performance Guarantee
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