Proteintech

NKCC1 Polyclonal Antibody

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Antibody Testing Data (5)

NKCC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 5

NKCC1 Antibody (13884-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using (Product # 13884-1-AP) (NKCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Product Image Gallery

NKCC1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 13884-1-AP) NKCC1 antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

NKCC1 Antibody in Immunoprecipitation (IP) — IP result of anti-NKCC1 (IP:13884-1-AP, 4ug; Detection:13884-1-AP 1:500) with mouse brain tissue lysate 3600ug.

Product Details

13884-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NKCC1 Fusion Protein Ag4991 (759-1150 aa encoded by BC033003)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: GSSTQALTY LNALQHSIRL SGVEDHVKNF RPQCLVMTGA PNSRPALLHL VHDFTKNVGL MICGHVHMGP RRQAMKEMSI DQAKYQRWLI KNKMKAFYAP VHADDLREGA QYLMQAAGLG RMKPNTLVLG FKKDWLQADM RDVDMYINLF HDAFDIQYGV VVIRLKEGLD ISHLQGQEEL LSSQEKSPGT KDVVVSVEYS KKSDLDTSKP LSEKPITHKV EEEDGKTATQ PLLKKESKGP IVPLNVADQK LLEASTQFQK KQGKNTIDVW WLFDDGGLTL LIPYLLTTKK KWKDCKIRVF IGGKINRIDH DRRAMATLLS KFRIDFSDIM VLGDINTKPK KENIIAFEEI IEPYRLHEDD KEQDIADKMK EDEPWRITDN ELELYKTKFY EPC (759-1150 aa encoded by B C033003)

Target Information

NKCC1 mediates sodium and chloride transport and reabsorption. NKCC1 is a membrane protein and is important in maintaining proper ionic balance and cell volume. NKCC1 is an electrically silent transporter system that mediates sodium and chloride reabsorption. Further, NKCC1 plays a vital role in the regulation of ionic balance and cell volume and is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for NKCC1. Diseases associated with SLC12A2 include Basosquamous Carcinoma and Metatypical Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Alterations in histology of the aging salivary gland and correlation with the glandular inflammatory microenvironment.

iScience

Li N,Ye Y,Wu Y,Li L,Hu J,Luo D,Li Y,Yang J,Gao Y,Hai W,Xie Y,Jiang L

Fri May 19 00:00:00 EDT 2023

Correlation of age at seizure onset with GABA_A receptor subunit and chloride Co-transporter configuration in Focal cortical dysplasia (FCD).

Neuroscience letters

Sharma D,Tripathi M,Doddamani R,Sharma MC,Lalwani S,Sarat Chandra P,Banerjee Dixit A,Banerjee J

Mon Feb 06 00:00:00 EST 2023

The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.

PLoS biology

Tóth K,Lénárt N,Berki P,Fekete R,Szabadits E,Pósfai B,Cserép C,Alatshan A,Benkő S,Kiss D,Hübner CA,Gulyás A,Kaila K,Környei Z,Dénes Á

Sat Jan 01 00:00:00 EST 2022

Atlas of human dental pulp cells at multiple spatial and temporal levels based on single-cell sequencing analysis.

Frontiers in physiology

Ren H,Wen Q,Zhao Q,Wang N,Zhao Y

Sat Oct 22 00:00:00 EDT 2022

Novel γ-sarcoglycan interactors in murine muscle membranes.

Skeletal muscle

Smith TC,Vasilakos G,Shaffer SA,Puglise JM,Chou CH,Barton ER,Luna EJ

Sat Jan 22 00:00:00 EST 2022

Novel γ-sarcoglycan interactors in murine muscle membranes.

Skeletal muscle

Smith TC,Vasilakos G,Shaffer SA,Puglise JM,Chou CH,Barton ER,Luna EJ

Sat Jan 22 00:00:00 EST 2022

Novel γ-sarcoglycan interactors in murine muscle membranes.

Skeletal muscle

Smith TC,Vasilakos G,Shaffer SA,Puglise JM,Chou CH,Barton ER,Luna EJ

Sat Jan 22 00:00:00 EST 2022

NKCC1 promotes proliferation, invasion and migration in human gastric cancer cells via activation of the MAPK-JNK/EMT signaling pathway.

Journal of Cancer

Wang JF,Zhao K,Chen YY,Qiu Y,Zhu JH,Li BP,Wang Z,Chen JQ

Tue Jan 05 00:00:00 EST 2021

NKCC1 promotes proliferation, invasion and migration in human gastric cancer cells via activation of the MAPK-JNK/EMT signaling pathway.

Journal of Cancer

Wang JF,Zhao K,Chen YY,Qiu Y,Zhu JH,Li BP,Wang Z,Chen JQ

Tue Jan 05 00:00:00 EST 2021

NKCC1 promotes proliferation, invasion and migration in human gastric cancer cells via activation of the MAPK-JNK/EMT signaling pathway.

Journal of Cancer

Wang JF,Zhao K,Chen YY,Qiu Y,Zhu JH,Li BP,Wang Z,Chen JQ

Tue Jan 05 00:00:00 EST 2021

WNK3 Promotes Neuronal Survival after Traumatic Brain Injury in Rats.

Neuroscience

Zhu J,Lin X,Chen C,Tan H,Gao Y,Li D,Chen G

Sun Nov 21 00:00:00 EST 2021

Parasympathectomy increases resting secretion of the submandibular gland in minipigs in the long term.

Journal of cellular physiology

Zhang XM,Huang Y,Cong X,Qu LH,Zhang K,Wu LL,Zhang Y,Yu GY

Sat Jun 01 00:00:00 EDT 2019

Parasympathectomy increases resting secretion of the submandibular gland in minipigs in the long term.

Journal of cellular physiology

Zhang XM,Huang Y,Cong X,Qu LH,Zhang K,Wu LL,Zhang Y,Yu GY

Sat Jun 01 00:00:00 EDT 2019

Early uneven ear input induces long-lasting differences in left-right motor function.

PLoS biology

Antoine MW,Zhu X,Dieterich M,Brandt T,Vijayakumar S,McKeehan N,Arezzo JC,Zukin RS,Borkholder DA,Jones SM,Frisina RD,Hébert JM

Thu Mar 01 00:00:00 EST 2018

Early uneven ear input induces long-lasting differences in left-right motor function.

PLoS biology

Antoine MW,Zhu X,Dieterich M,Brandt T,Vijayakumar S,McKeehan N,Arezzo JC,Zukin RS,Borkholder DA,Jones SM,Frisina RD,Hébert JM

Thu Mar 01 00:00:00 EST 2018

The Severity of Vestibular Dysfunction in Deafness as a Determinant of Comorbid Hyperactivity or Anxiety.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Antoine MW,Vijayakumar S,McKeehan N,Jones SM,Hébert JM

Wed May 17 00:00:00 EDT 2017

The Severity of Vestibular Dysfunction in Deafness as a Determinant of Comorbid Hyperactivity or Anxiety.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Antoine MW,Vijayakumar S,McKeehan N,Jones SM,Hébert JM

Wed May 17 00:00:00 EDT 2017

The Severity of Vestibular Dysfunction in Deafness as a Determinant of Comorbid Hyperactivity or Anxiety.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Antoine MW,Vijayakumar S,McKeehan N,Jones SM,Hébert JM

Wed May 17 00:00:00 EDT 2017

The Severity of Vestibular Dysfunction in Deafness as a Determinant of Comorbid Hyperactivity or Anxiety.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Antoine MW,Vijayakumar S,McKeehan N,Jones SM,Hébert JM

Wed May 17 00:00:00 EDT 2017

Discovery of NKCC1 as a potential therapeutic target to inhibit hepatocellular carcinoma cell growth and metastasis.

Oncotarget

Zhou Y,Sun W,Chen N,Xu C,Wang X,Dong K,Zhang B,Zhang J,Hao N,Sun A,Wei H,He F,Jiang Y

Tue Sep 12 00:00:00 EDT 2017

Regulation of GABA Equilibrium Potential by mGluRs in Rat Hippocampal CA1 Neurons.

PloS one

Yang B,Rajput PS,Kumar U,Sastry BR

Tue May 31 00:00:00 EDT 2016

Regulation of GABA Equilibrium Potential by mGluRs in Rat Hippocampal CA1 Neurons.

PloS one

Yang B,Rajput PS,Kumar U,Sastry BR

Tue May 31 00:00:00 EDT 2016

Bile deficiency induces changes in intestinal Cl(-) and HCO3 (-) secretions in mice.

Acta physiologica (Oxford, England)

Song P,Du Y,Song W,Liu X,Hong L,Li H,Xie H,Zhou L,Tuo B,Zheng S

Sun Jun 01 00:00:00 EDT 2014

Bile deficiency induces changes in intestinal Cl(-) and HCO3 (-) secretions in mice.

Acta physiologica (Oxford, England)

Song P,Du Y,Song W,Liu X,Hong L,Li H,Xie H,Zhou L,Tuo B,Zheng S

Sun Jun 01 00:00:00 EDT 2014

A causative link between inner ear defects and long-term striatal dysfunction.

Science (New York, N.Y.)

Antoine MW,Hübner CA,Arezzo JC,Hébert JM

Fri Sep 06 00:00:00 EDT 2013

A causative link between inner ear defects and long-term striatal dysfunction.

Science (New York, N.Y.)

Antoine MW,Hübner CA,Arezzo JC,Hébert JM

Fri Sep 06 00:00:00 EDT 2013

A causative link between inner ear defects and long-term striatal dysfunction.

Science (New York, N.Y.)

Antoine MW,Hübner CA,Arezzo JC,Hébert JM

Fri Sep 06 00:00:00 EDT 2013

A causative link between inner ear defects and long-term striatal dysfunction.

Science (New York, N.Y.)

Antoine MW,Hübner CA,Arezzo JC,Hébert JM

Fri Sep 06 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: Basolateral Na-K-Cl symporter; bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2; protein phosphatase 1, regulatory subunit 141; sodium/potassium/chloride cotransporters; sodium/potassium/chloride transporters; solute carrier family 12 (sodium/potassium/chloride transporter), member 2; solute carrier family 12 (sodium/potassium/chloride transporters), member 2; Solute carrier family 12 member 2; solute carrier family 12, member 2

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Gene Aliases: 9330166H04Rik; BSC; BSC2; mBSC2; NKCC1; PPP1R141; SLC12A2; sy-ns

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UniProt ID: (Human) P55011, (Mouse) P55012

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Entrez Gene ID: (Human) 6558, (Rat) 83629, (Mouse) 20496

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Molecular Function: secondary carrier transporter

Function(s)

transport ion transport potassium ion transport sodium ion transport ammonium transport transepithelial chloride transport transepithelial ammonium transport ammonium transmembrane transport chloride transmembrane transport chloride transport hyperosmotic response gamma-aminobutyric acid signaling pathway aging multicellular organism growth positive regulation of cell volume detection of mechanical stimulus involved in sensory perception of sound branching involved in mammary gland duct morphogenesis mammary duct terminal end bud growth organic cation transport transmembrane transport

Process(es)

protein binding sodium:potassium:chloride symporter activity ammonium transmembrane transporter activity cation:chloride symporter activity transporter activity symporter activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NKCC1 Polyclonal Antibody

NKCC1 Antibody (13884-1-AP) in IHC (P)

Product Details

13884-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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