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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
0.04-0.4 µg/mL | - |
Immunohistochemistry (IHC) |
1:50-1:200 | - |
Immunocytochemistry (ICC/IF) |
0.25-2 µg/mL | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant protein corresponding to Human NLE1 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.2 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.2, with 40% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2644710 |
Immunogen sequence: TIKVWRAHDG VLCRTLQGHG HWVNTMALST DYALRTGAFE PAEASVNPQD LQGSLQELKE RALSRYNLVR GQGPERLVSG SDDFTL
Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 95%.
NLE1 plays a role in regulating Notch activity, in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. NLE1 is required during embryogenesis for inner mass cell survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: FLJ10458; Notchless gene homolog; Notchless protein homolog 1; novel WD40 repeat containing protein
Gene Aliases: AL022765; BC018399; HUSSY-07; l11Jus1; l11Jus4; Nle; NLE1; RGD1308301
UniProt ID: (Human) Q9NVX2, (Mouse) Q8VEJ4
Entrez Gene ID: (Human) 54475, (Rat) 303372, (Mouse) 217011
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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