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NLRP3 Polyclonal Antibody
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FIGURE: 1 / 4
NLRP3 Antibody (600-401-R14) in IHC (P)
Immunohistochemistry analysis of NLRP3 using anti-NLRP3 antibody. NLRP3 was detected in a paraffin-embedded section of human squamous cell cervical carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-NLRP3 Antibody overnight at 4°... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
600-401-R14
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
NLRP3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the N-terminal of human NLRP3.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.5 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome, and neonatal-onset multisystem inflammatory disease. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: caterpiller protein 1.1; cold auto-inflammatory syndrome 1; PYPAF1; NALP3; C1ORF7; cold autoinflammatory syndrome 1 homolog; cold autoinflammatory syndrome 1 protein; cold autoinflammatory syndrome 1 protein homolog; cold-induced autoinflammatory syndrome 1 protein; cryopyrin; cryopyrin, NACHT, LRR and PYD domains - containing protein 3; deafness, autosomal dominant 34; FLJ95925; mast cell maturation-associated-inducible protein 1; member of the NALP family of inflammatory mediators; MMIG-1; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; NACHT/LRR/pyrin domain-containing protein 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; PYD, NACHT, and LRR containing protein; PYRIN-containing APAF1-like protein 1; unnamed protein product
Gene Aliases: AGTAVPRL; AII; AII/AVP; AVP; C1orf7; CIAS1; CLR1.1; DFNA34; FCAS; FCAS1; FCU; KEFH; Mmig1; MWS; NALP3; PYPAF1
UniProt ID: (Human) Q96P20, (Mouse) Q8R4B8, (Rat) D4A523
Entrez Gene ID: (Human) 114548, (Mouse) 216799, (Rat) 287362
protein binding
ATP binding
ATPase activity
protein binding, bridging
signaling adaptor activity
identical protein binding
peptidoglycan binding
ADP binding
sequence-specific DNA binding
binding, bridging
phosphatidylinositol-4-phosphate binding
DNA-binding transcription factor binding
small molecule sensor activity
cysteine-type endopeptidase activator activity
molecular condensate scaffold activity
phosphatidylinositol phosphate binding
pattern recognition receptor signaling pathway
acute inflammatory response
negative regulation of acute inflammatory response
positive regulation of cytokine production involved in immune response
positive regulation of type 2 immune response
inflammatory response
osmosensory signaling pathway
protein secretion
detection of biotic stimulus
response to virus
negative regulation of interleukin-1 beta production
positive regulation of interleukin-1 beta production
positive regulation of interleukin-13 production
positive regulation of interleukin-18 production
positive regulation of interleukin-4 production
positive regulation of interleukin-5 production
interleukin-18-mediated signaling pathway
NLRP3 inflammasome complex assembly
response to ethanol
positive regulation of T-helper 2 cell differentiation
positive regulation of transcription from RNA polymerase II promoter
regulation of inflammatory response
negative regulation of inflammatory response
positive regulation of inflammatory response
defense response to Gram-positive bacterium
protein homooligomerization
protein maturation
defense response to virus
pyroptosis
interleukin-1-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to peptidoglycan
cellular response to virus
positive regulation of NLRP3 inflammasome complex assembly
negative regulation of NIK/NF-kappaB signaling
positive regulation of NIK/NF-kappaB signaling
positive regulation of T-helper 17 cell differentiation
positive regulation of T-helper 2 cell cytokine production
apoptotic process
defense response
signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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