Immunohistochemistry using NMDA Receptor Subunit 2A Polyclonal Antibody, Rabbit: Rat brain cryosections labeled with anti-NMDA receptor, subunit 2A (rat), rabbit IgG fraction (Cat. no.A6473) and detected using Alexa Fluor® 488 goat anti–rabbit IgG antibody (Cat. no. A11008). The tissue was also labeled with Alexa Fluor® 594 anti–glial fibrillary acidic protein antibody (Cat. no.A21295) and counterstained with TOTO®-3 iodide (Cat. no.T3604), which was pseudocolored light blue in this image.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein containing amino acid residues 1253–1391 of the C-terminal region of rat brain subunit 2A|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:500-1:5000|
|Immunoprecipitation (IP)||3 µL|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes the 180 kDa 2A subunit from rat, mouse and human, and does not cross-react with the 2B or 2C subunits.
Storage and reconstitution: when stored at -20°C or below, the lyophilized antibody should retain full activity for over one year. Reconstitute using 50 µL PBS, pH 7.4, to yield a 0.2 mg/mL solution. If storing at 0-4°C, add sodium azide to a final concentration of 2mM. For longer storage, divide the solution into aliquots and freeze at -20°C, avoiding freeze/thaw cycles.
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mechanisms of Functional Hypoconnectivity in the Medial Prefrontal Cortex of Mecp2 Null Mice.
A-6473 was used in western blot to investigate how the loss of Mecp2 disrupts function in the murine medial prefrontal cortex using acute brain slices and behavioral testing.
|Sceniak MP,Lang M,Enomoto AC,Howell CJ,Hermes DJ,Katz DM||Cerebral cortex (New York, N.Y. : 1991) (26:1938)||2016|
Chronic copper exposure causes spatial memory impairment, selective loss of hippocampal synaptic proteins, and activation of PKR/eIF2α pathway in mice.
A-6473 was used in western blot to study the effect of copper exposure on memory
|Ma Q,Ying M,Sui X,Zhang H,Huang H,Yang L,Huang X,Zhuang Z,Liu J,Yang X||Journal of Alzheimer's disease : JAD (43:1413)||2014|
PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors.
A-6473 was used in immunocytochemistry and western blot to study the roles of the NR2A and NR2B subunits, and of their scaffolding proteins, in remodeling the dendritic architecture of developing hippocampal neurons
|Bustos FJ,Varela-Nallar L,Campos M,Henriquez B,Phillips M,Opazo C,Aguayo LG,Montecino M,Constantine-Paton M,Inestrosa NC,van Zundert B||PloS one (9:null)||2014|
Estradiol potentiation of NR2B-dependent EPSCs is not due to changes in NR2B protein expression or phosphorylation.
A-6473 was used in western blot to investigate the mechanism for the synaptic regulation by estradiol
|Snyder MA,Cooke BM,Woolley CS||Hippocampus (21:398)||2011|
Antibody-mediated targeted gene transfer of helper virus-free HSV-1 vectors to rat neocortical neurons that contain either NMDA receptor 2B or 2A subunits.
A-6473 was used in immunohistochemistry to investigate antibody-mediated targeted gene transfer.
|Cao H,Zhang GR,Geller AI||Brain research (1415:127)||2011|
Cellular and synaptic mechanisms of anti-NMDA receptor encephalitis.
A-6473 was used in western blot to investigate the effects of autoantibodies to the NMDA receptor
|Hughes EG,Peng X,Gleichman AJ,Lai M,Zhou L,Tsou R,Parsons TD,Lynch DR,Dalmau J,Balice-Gordon RJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:5866)||2010|
Neuronal interleukin-16 (NIL-16): a dual function PDZ domain protein.
A-6473 was used in immunocytochemistry to investigate the role of IL-16 in the nervous system.
|Kurschner C,Yuzaki M||The Journal of neuroscience : the official journal of the Society for Neuroscience (19:7770)||1999|