Invitrogen

NMDAR1 Polyclonal Antibody

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NMDAR1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of NMDA Receptor 1 was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NMDA Receptor 1 Rabbit Polyclonal Antibody (Product # PA3-102) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

NMDAR1 Antibody in Immunohistochemistry (IHC) — Immunofluorescence image of NMDA receptor type 1 in rat brain tissue using Product # PA3-102.

NMDAR1 Antibody in Western Blot (WB) — Western blot was performed using Anti-NMDAR1 Polyclonal Antibody (Product # PA3-102) and a 110kDa band corresponding to NMDAR1 was observed across cell lines and tissues except liver and kidney tissues. Membrane enriched extracts (30 µg lysate) of (Fig. a) SH-SY5Y (Lane 1), U-87 MG (Lane 2), Neuro-2a (Lane 3), HeLa (Lane 4), (Fig. b) Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Liver (Lane 3), Rat Liver (Lane 4), Mouse Kidney (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:600 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:6000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076). Few uncharacterized bands were observed between 30-50 kDa.

NMDAR1 Antibody in Flow Cytometry (Flow) — Fig 5 CSF-1 regulates cell surface NMDA-R in macrophages. Cell surface NMDA-R was labeled in BMDMs and PMs using NR1 subunit-specific primary antibody and Alexa Fluor 647-conjugated secondary antibody. (A) Representative flow cytometry histograms are shown for BMDMs, quiescent PMs (basal), PMs treated with L929 cell-conditioned medium for 5 days (L929), and PMs treated with CSF-1 (10 ng/mL) for 5 days. As a control, PMs were treated with secondary antibody alone (2deg only). The bar graph (right panel) shows the fold increase in the MPF compared with quiescent PMs (no exposure to L929 cell conditioned medium or CSF-1) (mean +- SEM, n = 4; one-way ANOVA with Bonferroni post-hoc test, **p<0.01, ***p<0.001). (B) Representative NMDA-R NR1 subunit flow cytometry histograms are shown for PMs treated with CSF-1 (10 ng/mL) for increasing periods of time (0, 1, 2, and 5 days). The fold increase in the MPF compared to untreated cells is presented (mean +- SEM, n = 4; one-way ANOVA with Bonferroni post-hoc test, **p<0.01, ***p<0.001, ns = not significant). (C) IF microscopy images showing NMDA-R NR1 subunit in fixed, non-permeabilized PMs cultured in basal medium (left) or in CSF-1-supplemented medium (right) for 2 days. Images are representative of 3 independent experiments (scale bar, 10 mum). The bar graph shows the quantification of mean pixel intensity as measured in 14 independent fields of view in three separate slides (mean +- SEM, unpaired t test, ***p<0.001).

Product Details

PA3-102

Applications

Tested Dilution

Publications

Western Blot (WB)

1:600

View 10 publications 10 publications

Immunohistochemistry (IHC)

1:200

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:250

Flow Cytometry (Flow)

View 2 publications 2 publications

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Guinea pig, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues (195)N Y E N L D Q L S Y D N K R G P(210) C of Exon 5 of rat NMDA Receptor Type 1 (Isoforms B, F, G).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2112003

Product Specific Information

PA3-102 detects N-methyl-D-aspartate (NMDA) receptor type 1.

PA3-102 has been used successfully in Western blot, ELISA, immunoprecipitation, immunohistochemistry, and immunocytochemistry procedures. In Western blot analysis of rat brain synaptic membranes this antibody detects a ~120 kDa protein representing NMDA receptor type 1.

The PA3-102 immunogen is a synthetic peptide corresponding to residues (195)N Y E N L D Q L S Y D N K R G P(210) C of Exon 5 of rat NMDA Receptor Type 1 (Isoforms B, F, G).

Target Information

NMDAR1 encodes a protein that is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Repeated Galvanic Vestibular Stimulation Modified the Neuronal Potential in the Vestibular Nucleus.

Neural plasticity

Kim G,Lee S,Kim KS

PA3-102 was used in Immunohistochemistry to show that AMPA and NMDA receptors decreased after the repeated stimuli by 28.32 and 16.09%, respectively, implying the modification in the neuronal amplitudes.

Wed Apr 07 00:00:00 EDT 2021

Glycogen synthase kinase-3 inhibition rescues sex-dependent contextual fear memory deficit in human immunodeficiency virus-1 transgenic mice.

British journal of pharmacology

Moidunny S,Benneyworth MA,Titus DJ,Beurel E,Kolli U,Meints J,Jalodia R,Ramakrishnan S,Atkins CM,Roy S

PA3-102 was used in Western Blot to investigate synaptic mechanisms associated with sex differences in HIV-associated neurocognitive disorders.

Tue Dec 01 00:00:00 EST 2020

Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.

Frontiers in cellular neuroscience

Lisek M,Ferenc B,Studzian M,Pulaski L,Guo F,Zylinska L,Boczek T

Published figure using NMDAR1 polyclonal antibody (Product # PA3-102) in Western Blot

Thu Oct 01 00:00:00 EDT 2020

SK Channel Modulates Synaptic Plasticity by Tuning CaMKIIα/β Dynamics.

Frontiers in synaptic neuroscience

Shrestha A,Sultana R,Lee CC,Ogundele OM

Published figure using NMDAR1 polyclonal antibody (Product # PA3-102) in Western Blot

Tue Sep 29 00:00:00 EDT 2020

Autism-Misregulated eIF4G Microexons Control Synaptic Translation and Higher Order Cognitive Functions.

Molecular cell

Gonatopoulos-Pournatzis T,Niibori R,Salter EW,Weatheritt RJ,Tsang B,Farhangmehr S,Liang X,Braunschweig U,Roth J,Zhang S,Henderson T,Sharma E,Quesnel-Vallières M,Permanyer J,Maier S,Georgiou J,Irimia M,Sonenberg N,Forman-Kay JD,Gingras AC,Collingridge GL,Woodin MA,Cordes SP,Blencowe BJ

PA3-102 was used in Western Blotting to reveal an autism-disrupted mechanism by which alternative splicing specializes neuronal translation to control higher order cognitive functioning.

Thu Mar 19 00:00:00 EDT 2020

Tissue-type plasminogen activator selectively inhibits multiple toll-like receptors in CSF-1-differentiated macrophages.

PloS one

Das L,Azmoon P,Banki MA,Mantuano E,Gonias SL

Published figure using NMDAR1 polyclonal antibody (Product # PA3-102) in Flow Cytometry

Wed Mar 18 00:00:00 EDT 2020

Synaptic proximity enables NMDAR signalling to promote brain metastasis.

Nature

Zeng Q,Michael IP,Zhang P,Saghafinia S,Knott G,Jiao W,McCabe BD,Galván JA,Robinson HPC,Zlobec I,Ciriello G,Hanahan D

PA3-102 was used in Immunohistochemistry to show that B2BM cells co-opt a neuronal signalling pathway that was recently implicated in invasive tumour growth, involving activation by glutamate ligands of N-methyl-D-aspartate receptors (NMDARs), which is key in model systems for metastatic colonization of the brain and is associated with poor prognosis.

Sun Sep 01 00:00:00 EDT 2019

Red and near-infrared light induces intracellular Ca²⁺ flux via the activation of glutamate N-methyl-D-aspartate receptors.

Journal of cellular physiology

Golovynska I,Golovynskyi S,Stepanov YV,Garmanchuk LV,Stepanova LI,Qu J,Ohulchanskyy TY

PA3-102 was used in Immunohistochemistry-immunofluorescence to demonstrate that NIR light can be used for nonthermal and nonpharmacological stimulation of NMDARs in cancer cells.

Sun Sep 01 00:00:00 EDT 2019

Hydrogen sulfide inhibits Ca²⁺-induced mitochondrial permeability transition pore opening in type-1 diabetes.

American journal of physiology. Endocrinology and metabolism

Papu John AS,Kundu S,Pushpakumar S,Amin M,Tyagi SC,Sen U

PA3-102 was used in Western Blot to show that NMDA-R1-mediated Ca2+ influx in diabetes induces MPTP opening via CypD activation leading to increased oxidative stress and renal injury, and H2S protects diabetic kidney from injury by blocking mitochondrial Ca2+ permeability through NMDA-R1 pathway.

Thu Aug 01 00:00:00 EDT 2019

Cereblon Maintains Synaptic and Cognitive Function by Regulating BK Channel.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Choi TY,Lee SH,Kim YJ,Bae JR,Lee KM,Jo Y,Kim SJ,Lee AR,Choi S,Choi LM,Bang S,Song MR,Chung J,Lee KJ,Kim SH,Park CS,Choi SY

PA3-102 was used in Western Blotting to investigate the role of cereblon in synaptic function and animal behaviour.

Wed Apr 04 00:00:00 EDT 2018

Autism-like behavior caused by deletion of vaccinia-related kinase 3 is improved by TrkB stimulation.

The Journal of experimental medicine

Kang MS,Choi TY,Ryu HG,Lee D,Lee SH,Choi SY,Kim KT

Published figure using NMDAR1 polyclonal antibody (Product # PA3-102) in Western Blot

Mon Oct 02 00:00:00 EDT 2017

Low levels of methyl β-cyclodextrin disrupt GluA1-dependent synaptic potentiation but not synaptic depression.

Journal of neurochemistry

Choi TY,Jung S,Nah J,Ko HY,Jo SH,Chung G,Park K,Jung YK,Choi SY

PA3-102 was used in western blot to study the effects of methyl beta-cyclodextrin on GluA1-dependent synaptic potentiation

Sun Feb 01 00:00:00 EST 2015

Sox18 preserves the pulmonary endothelial barrier under conditions of increased shear stress.

Journal of cellular physiology

Gross CM,Aggarwal S,Kumar S,Tian J,Kasa A,Bogatcheva N,Datar SA,Verin AD,Fineman JR,Black SM

Published figure using NMDAR1 polyclonal antibody (Product # PA3-102) in Western Blot

Sat Nov 01 00:00:00 EDT 2014

Hippocampal long-term potentiation is disrupted during expression and extinction but is restored after reinstatement of morphine place preference.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Portugal GS,Al-Hasani R,Fakira AK,Gonzalez-Romero JL,Melyan Z,McCall JG,Bruchas MR,Morón JA

PA3-102 was used in western blot to study the reinstatement of morphine conditioned place preference and the role of hippocampal synaptic plasticity and NMDAR expression

Wed Jan 08 00:00:00 EST 2014

Hijacking the neuronal NMDAR signaling circuit to promote tumor growth and invasion.

Cell

Li L,Hanahan D

PA3-102 was used in flow cytometry and immunohistochemistry to study the role of NMDAR signaling in tumorigenesis using a murine model

Thu Mar 28 00:00:00 EDT 2013

Impacts of forebrain neuronal glycine transporter 1 disruption in the senescent brain: evidence for age-dependent phenotypes in Pavlovian learning.

Behavioral neuroscience

Dubroqua S,Singer P,Boison D,Feldon J,Möhler H,Yee BK

PA3-102 was used in immunohistochemistry to investigate the role of forebrain neuronal glycine transporter 1 in cognitive functions

Wed Dec 01 00:00:00 EST 2010

Bioinformatics

Protein Aliases: GluN1; Glun1r; glutamate [NMDA] receptor subunit zeta 1; Glutamate [NMDA] receptor subunit zeta-1; glutamate receptor; Glutamate receptor ionotropic, NMDA 1; glutamate receptor, ionotropic, N-methyl D-aspartate 1; N-methyl-D-aspartate glutamate receptor; N-methyl-D-aspartate receptor channel, subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; neurotransmitter receptor; NMD-R1; NMDA R1 receptor C1 cassette; NMDA receptor 1; NMDZ1; NMZ1; NR1C1; NR1C2; NR1C2'; NR1N1; pGluN1; RP11-350O14.1

Gene Aliases: GluN1; GluRdelta1; Glurz1; GluRzeta1; GRIN1; M100174; MRD8; NMD-R1; NMDA1; Nmdar; NMDAR1; NR1; Rgsc174

UniProt ID: (Human) Q05586, (Rat) P35439, (Mouse) P35438

Entrez Gene ID: (Human) 2902, (Rat) 24408, (Mouse) 14810

Molecular Function: transmembrane signal receptor