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      NMDAR2A Polyclonal Antibody

      See 7 other NMDAR2A antibodies ›
      NMDAR2A Polyclonal Antibody for Western Blot, IF, IHC (F), IP and ELISA

      Catalog#: A-6473 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      A-6473 10 µg
      392.00
      Please enter a quantity in the range of 1 to 199.
      Suggested Secondary Antibodies

      Anti-Rabbit Secondary Antibodies (264)
      Alexa Fluor® Anti-Rabbit Secondary Antibodies (69)
      HRP Anti-Rabbit Secondary Antibodies (30)

      Product Details

      References

      Bioinformatics

      Documents
      Details
      Tested species reactivity Human, Mouse, Rat
      Published species reactivity Rat, Mouse, Human, Not Applicable
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen Fusion protein containing amino acid residues 1253–1391 of the C-terminal region of rat brain subunit 2A
      Conjugate Unconjugated
      Form Lyophilized
      Purification IgG fraction
      Storage buffer PBS
      Contains no preservative
      Storage Conditions -20°C
      Tested Applications Dilution *
      ELISA (ELISA) Assay Dependent
      Immunofluorescence (IF) 1:500-1:5000
      Immunohistochemistry (Frozen) (IHC (F)) 1:500-1:5000
      Immunoprecipitation (IP) 3 µL
      Western Blot (WB) 1:100-1:500

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Published Applications
      Western Blot (WB) See 5 publications below
      Immunohistochemistry (IHC) See 1 publications below
      Miscellaneous PubMed (MISC) See 2 publications below

      Product Specific Information

      This antibody recognizes the 180 kDa 2A subunit from rat, mouse and human, and does not cross-react with the 2B or 2C subunits.

      Storage and reconstitution: when stored at -20°C or below, the lyophilized antibody should retain full activity for over one year. Reconstitute using 50 µL PBS, pH 7.4, to yield a 0.2 mg/mL solution. If storing at 0-4°C, add sodium azide to a final concentration of 2mM. For longer storage, divide the solution into aliquots and freeze at -20°C, avoiding freeze/thaw cycles.

      Background/Target Information

      N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate-gated ion channels. These receptors have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References

      Western Blot

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Mouse 1:500 SALM4 suppresses excitatory synapse development by cis-inhibiting trans-synaptic SALM3-LAR adhesion.
      A-6473 was used in western blot propose that SALM4 negatively regulates excitatory synapses via inhibition of SALM3-LAR adhesion       		Lie E,Ko JS,Choi SY,Roh JD,Cho YS,Noh R,Kim D,Li Y,Kang H,Choi TY,Nam J,Mah W,Lee D,Lee SG,Kim HM,Kim H,Choi SY,Um JW,Kang MG,Bae YC,Ko J,Kim E Nature communications (7:null) 2016
      Mouse 1:500 Mechanisms of Functional Hypoconnectivity in the Medial Prefrontal Cortex of Mecp2 Null Mice.
      A-6473 was used in western blot to investigate how the loss of Mecp2 disrupts function in the murine medial prefrontal cortex using acute brain slices and behavioral testing.       		Sceniak MP,Lang M,Enomoto AC,James Howell C,Hermes DJ,Katz DM Cerebral cortex (New York, N.Y. : 1991) (26:1938) 2016
      Mouse 1:200 Chronic copper exposure causes spatial memory impairment, selective loss of hippocampal synaptic proteins, and activation of PKR/eIF2α pathway in mice.
      A-6473 was used in western blot to study the effect of copper exposure on memory       		Ma Q,Ying M,Sui X,Zhang H,Huang H,Yang L,Huang X,Zhuang Z,Liu J,Yang X Journal of Alzheimer's disease : JAD (43:1413) 2014
      Rat 1:500 PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors.
      A-6473 was used in immunocytochemistry and western blot to study the roles of the NR2A and NR2B subunits, and of their scaffolding proteins, in remodeling the dendritic architecture of developing hippocampal neurons       		Bustos FJ,Varela-Nallar L,Campos M,Henriquez B,Phillips M,Opazo C,Aguayo LG,Montecino M,Constantine-Paton M,Inestrosa NC,van Zundert B PloS one (9:null) 2014
      Not Applicable 1:10,000 Estradiol potentiation of NR2B-dependent EPSCs is not due to changes in NR2B protein expression or phosphorylation.
      A-6473 was used in western blot to investigate the mechanism for the synaptic regulation by estradiol       		Snyder MA,Cooke BM,Woolley CS Hippocampus (21:398) 2011

      Immunohistochemistry

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Human Not Cited Antibody-mediated targeted gene transfer of helper virus-free HSV-1 vectors to rat neocortical neurons that contain either NMDA receptor 2B or 2A subunits.
      A-6473 was used in immunohistochemistry to investigate antibody-mediated targeted gene transfer.       		Cao H,Zhang GR,Geller AI Brain research (1415:127) 2011

      Miscellaneous PubMed

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Human 1:500 Cellular and synaptic mechanisms of anti-NMDA receptor encephalitis.
      A-6473 was used in western blot to investigate the effects of autoantibodies to the NMDA receptor       		Hughes EG,Peng X,Gleichman AJ,Lai M,Zhou L,Tsou R,Parsons TD,Lynch DR,Dalmau J,Balice-Gordon RJ The Journal of neuroscience : the official journal of the Society for Neuroscience (30:5866) 2010
      Mouse 1:500 Neuronal interleukin-16 (NIL-16): a dual function PDZ domain protein.
      A-6473 was used in immunocytochemistry to investigate the role of IL-16 in the nervous system.       		Kurschner C,Yuzaki M The Journal of neuroscience : the official journal of the Society for Neuroscience (19:7770) 1999

      Bioinformatics

      Protein Aliases:

      GluN2A; GluRepsilon1; Glutamate [NMDA] receptor subunit epsilon-1; Glutamate NMDA receptor subunit epsilon-1; Glutamate receptor ionotropic NMDA 2A; Glutamate receptor ionotropic, NMDA 2A; glutamate receptor, ionotropic, N-methyl D-aspartate 2A; Grin2a; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor channel, subunit epsilon-1; N-methyl-D-aspartate receptor subunit 2A; N-Methyl-D-Aspartate Receptor Type 2A; NMDAR2A; NR2A

      Gene Aliases:

      EPND; FESD; GluN2A; GRIN2A; LKS; NMDAR2A; NR2A

      UniProt ID:

      (Human) Q12879, (Mouse) P35436, (Rat) Q00959

      Entrez Gene ID:

      (Human) 2903, (Mouse) 14811, (Rat) 24409

      Molecular Function:

      ion channel ionotropic glutamate receptor ligand-gated ion channel receptor transporter

      Function(s) Process(es)

      Documents

      Material safety data sheets (MSDS)

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