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Invitrogen
NMDAR2B Polyclonal Antibody
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FIGURE: 1 / 9
NMDAR2B Antibody (A-6474) in WB
Western blot was performed using NMDAR2B Polyclonal Antibody (Product # A-6474) and a 166 kDa and 170 kDa band corresponding to NMDAR2B were observed across tissues tested except for mouse liver and heart, which are the low expressors for NMDAR2B. Whole cell extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Pup Brain (Lane 2), Mouse Cerebellum (Lane 3), Mouse Liver (Lane 4), Mouse Heart (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a ... View More
Product Details
A-6474
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Fusion protein containing amino acid residues 984-1104 of the C-terminal region of rat brain subunit 2B
Conjugate
Form
Lyophilized
Amount
10 µg
Purification
IgG fraction
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is specific for the ~180 kDa 2B subunit from rat, mouse and human.
Storage and reconstitution: when stored at -20°C or below, the lyophilized antibody should retain full activity for over one year. Reconstitute using 50 µL PBS, pH 7.4, to yield a 0.2 mg/mL solution. If storing at 0-4°C, add sodium azide to a final concentration of 2mM. For longer storage, divide the solution into aliquots and freeze at -20°C, avoiding freeze/thaw cycles.
Target Information
NMDAR2B is a member of the N-methyl-D-aspartate (NMDA) class of ionotropic glutamate receptors. NMDA receptors (NMDAR) have been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDAR play a role in neuronal development and has been implicated in several disorders of the central nervous system including Alzheimer's Disease, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity. NMDR2B is a heterotrimer comprised of seven enetically encoded and differentially expressed subunits that include NR1, NR2 and NR3. Diseases associated with the dysfunction of NMDAR2B include epileptic encephalopathy, autosomal dominant 6-related mental retardation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GluN2B; GluN2B(alt_5'UTR); GluRepsilon2; Glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; glutamate receptor, ionotropic, NMDA2B; GRIN 2B; HGNC:4586; hNR 3; MGC142178; MGC142180; N-methyl D-aspartate receptor 2B; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDAR2B; NMDE2; NME2; NR2B; NR3
Gene Aliases: AW490526; DEE27; EIEE27; GluN2B; GRIN2B; hNR3; MRD6; NMDAR2B; NR2B; NR3
UniProt ID: (Human) Q13224, (Mouse) Q01097, (Rat) Q00960
Entrez Gene ID: (Human) 2904, (Mouse) 14812, (Rat) 24410
beta-amyloid binding
NMDA glutamate receptor activity
ion channel activity
protein binding
zinc ion binding
ligand-gated ion channel activity
glycine binding
glutamate binding
glutamate-gated calcium ion channel activity
signaling receptor activity
metal ion binding
ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential
transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential
receptor activity
ionotropic glutamate receptor activity
receptor binding
interleukin-1 receptor binding
extracellular-glutamate-gated ion channel activity
cation channel activity
calcium channel activity
beta-catenin binding
drug binding
D2 dopamine receptor binding
ionotropic glutamate receptor binding
neurotransmitter binding
protein heterodimerization activity
cell adhesion molecule binding
transmembrane signal receptor
ion transport
glutamate receptor signaling pathway
chemical synaptic transmission
brain development
learning or memory
calcium-mediated signaling
ion transmembrane transport
ionotropic glutamate receptor signaling pathway
synaptic transmission, glutamatergic
regulation of membrane potential
response to ethanol
regulation of synaptic plasticity
regulation of neuronal synaptic plasticity
protein heterotetramerization
positive regulation of synaptic transmission, glutamatergic
excitatory postsynaptic potential
long-term synaptic potentiation
calcium ion transmembrane import into cytosol
cation transmembrane transport
excitatory chemical synaptic transmission
regulation of presynaptic membrane potential
negative regulation of dendritic spine maintenance
regulation of cation transmembrane transport
positive regulation of excitatory postsynaptic potential
action potential
behavioral fear response
in utero embryonic development
startle response
suckling behavior
transport
cation transport
calcium ion transport
sensory organ development
learning
memory
associative learning
embryo development
regulation of protein kinase A signaling
positive regulation of cell death
positive regulation of glutamate secretion
fear response
receptor clustering
regulation of MAPK cascade
regulation of long-term neuronal synaptic plasticity
behavioral response to pain
rhythmic process
positive regulation of synaptic transmission
detection of mechanical stimulus involved in sensory perception of pain
regulation of postsynaptic membrane potential
calcium ion transmembrane transport
response to amphetamine
long-term memory
response to mechanical stimulus
response to toxic substance
response to carbohydrate
response to manganese ion
cellular response to magnesium starvation
response to organic cyclic compound
response to amine
hippocampus development
cerebral cortex development
response to magnesium ion
multicellular organismal response to stress
response to cytokine
response to cocaine
response to calcium ion
response to methylmercury
response to electrical stimulus
response to other organism
response to growth hormone
response to fungicide
cellular response to amino acid stimulus
cellular response to manganese ion
cellular response to dsRNA
cellular response to growth factor stimulus
cellular response to lipid
cellular response to organic cyclic compound
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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