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|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein containing amino acid residues 984–1104 of the C-terminal region of rat brain subunit 2B|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:500-1:5000|
|Immunoprecipitation (IP)||3 µL|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
This antibody is specific for the ~180 kDa 2B subunit from rat, mouse and human.
Storage and reconstitution: when stored at -20°C or below, the lyophilized antibody should retain full activity for over one year. Reconstitute using 50 µL PBS, pH 7.4, to yield a 0.2 mg/mL solution. If storing at 0-4°C, add sodium azide to a final concentration of 2mM. For longer storage, divide the solution into aliquots and freeze at -20°C, avoiding freeze/thaw cycles.
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mechanisms of Functional Hypoconnectivity in the Medial Prefrontal Cortex of Mecp2 Null Mice.
A-6474 was used in western blot to investigate how the loss of Mecp2 disrupts function in the murine medial prefrontal cortex using acute brain slices and behavioral testing.
|Sceniak MP,Lang M,Enomoto AC,James Howell C,Hermes DJ,Katz DM||Cerebral cortex (New York, N.Y. : 1991) (26:1938)||2016|
Chronic copper exposure causes spatial memory impairment, selective loss of hippocampal synaptic proteins, and activation of PKR/eIF2α pathway in mice.
A-6474 was used in western blot to study the effect of copper exposure on memory
|Ma Q,Ying M,Sui X,Zhang H,Huang H,Yang L,Huang X,Zhuang Z,Liu J,Yang X||Journal of Alzheimer's disease : JAD (43:1413)||2014|
PSD95 suppresses dendritic arbor development in mature hippocampal neurons by occluding the clustering of NR2B-NMDA receptors.
A-6474 was used in immunocytochemistry and western blot to study the roles of the NR2A and NR2B subunits, and of their scaffolding proteins, in remodeling the dendritic architecture of developing hippocampal neurons
|Bustos FJ,Varela-Nallar L,Campos M,Henriquez B,Phillips M,Opazo C,Aguayo LG,Montecino M,Constantine-Paton M,Inestrosa NC,van Zundert B||PloS one (9:null)||2014|
Subunit-specific modulation of [(3)H]MK-801 binding to NMDA receptors mediated by dopamine receptor ligands in rodent brain.
A-6474 was used in western blot to identify DR ligands that directly modulate NMDARs.
|Wigestrand MB,Fonnum F,Ivar Walaas S||Neurochemistry international (61:266)||2012|
GluN2B; GluRepsilon2; Glutamate [NMDA] receptor subunit epsilon 2 precursor; Glutamate [NMDA] receptor subunit epsilon-2; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; glutamate receptor, ionotropic, NMDA2B; GRIN2B; hNR3; N-methyl D-aspartate receptor 2B; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDAR2B; NMDE2; NME2; NR2B; NR3
AW490526; EIEE27; GluN2B; GRIN2B; hNR3; MRD6; NMDAR2B; NR2B