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NME1 Antibody (PA5-79742) in ICC/IF
Immunofluorescence analysis of NME1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NME1 Polyclonal Antibody (Product # PA5-79742) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More
Product Details
PA5-79742
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human NM23A (137-152aa EELVDYTSCAQNWIYE).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2746857
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: expressed in non-metastatic cells 1 protein; expressed in non-metastatic cells 1 protein (NM23A) (nucleoside diphosphate kinase); expressed in non-metastatic cells 1, protein; expressed in non-metastatic cells 1, protein (NM23A) (nucleoside diphosphate kinase); GAAD; Granzyme A activated DNase (GAAD); Granzyme A-activated DNase; GZMA activated DNase; included; Metastasis inhibition factor NM23; NDK A; NDP kinase A; NDP kinase beta; NDPK-A; NM23-H1; nm23-M1; NM23H1B; NME1-NME2 spliced read-through transcript; non-metastatic cells 1, protein (NM23A) expressed in; Nucleoside diphosphate kinase A; nucleoside-diphosphate kinase 1; nucleotide diphosphate kinase; nucloside diphosphate kinase; Tumor metastatic process-associated protein
Gene Aliases: AL024257; AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1; NM23-M1; NM23A; NME1
UniProt ID: (Human) P15531, (Mouse) P15532, (Rat) Q05982
Entrez Gene ID: (Human) 4830, (Mouse) 18102, (Rat) 191575
magnesium ion binding
RNA polymerase II regulatory region sequence-specific DNA binding
single-stranded DNA binding
deoxyribonuclease activity
nucleoside diphosphate kinase activity
protein binding
ATP binding
GTP binding
intermediate filament binding
protein kinase binding
identical protein binding
gamma-tubulin binding
ribosomal small subunit binding
poly(A) RNA binding
nucleotide binding
kinase activity
transferase activity
enzyme binding
metal ion binding
kinase
negative regulation of myeloid leukocyte differentiation
purine nucleotide metabolic process
nucleoside diphosphate phosphorylation
GTP biosynthetic process
pyrimidine nucleotide metabolic process
UTP biosynthetic process
CTP biosynthetic process
DNA metabolic process
endocytosis
lactation
negative regulation of cell proliferation
nucleoside triphosphate biosynthetic process
negative regulation of gene expression
positive regulation of neuron projection development
response to amine
nucleobase-containing small molecule interconversion
hippocampus development
response to testosterone
cellular response to drug
regulation of apoptotic process
positive regulation of DNA binding
positive regulation of epithelial cell proliferation
response to cAMP
cellular response to glucose stimulus
cellular response to fatty acid
nervous system development
nucleotide metabolic process
phosphorylation
cell differentiation
mammary gland development
nucleic acid phosphodiester bond hydrolysis
DNA catabolic process
integrin-mediated signaling pathway
response to drug
negative regulation of apoptotic process
positive regulation of keratinocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
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