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Invitrogen
NME1 Polyclonal Antibody
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FIGURE: 1 / 3
NME1 Antibody (PA5-87395) in IHC (P)
Immunohistochemistry analysis of NME1 in paraffin-embedded mouse kidney. Samples were incubated with NME1 Polyclonal antibody (Product # PA5-87395) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-87395
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 77-177 of human NME1/NM23A (NP_9378181)
Conjugate
Form
Liquid
Concentration
0.61 mg/mL
Amount
61 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: A-549, MCF7, HeLa, HepG2; Cellular Location: Cytoplasm, Nucleus
Immunogen sequence: YVDLKDRPFF AGLVKYMHSG PVVAMVWEGL NVVKTGRVML GETNPADSKP GTIRGDFCIQ VGRNIIHGSD SVESAEKEIG LWFHPEELVD YTSCAQNWIY E
Target Information
NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; expressed in non-metastatic cells 1 protein; expressed in non-metastatic cells 1 protein (NM23A) (nucleoside diphosphate kinase); expressed in non-metastatic cells 1, protein; expressed in non-metastatic cells 1, protein (NM23A) (nucleoside diphosphate kinase); Farnesyl diphosphate kinase NME1; GAAD; Granzyme A activated DNase (GAAD); granzyme A-activated DNase; GZMA activated DNase; Histidine protein kinase NDK; Histidine protein kinase NME1; included; Metastasis inhibition factor NM23; NDK A; NDK1; NDP kinase A; NDP kinase beta; NDPK-A; NM23-H1; nm23-M1; NM23H1; NM23H1B; NME1; NME1-NME2 spliced read-through transcript; non-metastatic cells 1, protein (NM23A) expressed in; Non-specific serine/threonine protein kinase NME1; Nucleoside diphoshate kinase 1; Nucleoside diphosphate kinase A; nucleoside-diphosphate kinase 1; nucleotide diphosphate kinase; nucloside diphosphate kinase; Putative 3'-5'-DNA exonuclease NDK1; Putative granzyme A-activated DNA endonuclease; Tumor metastatic process-associated protein; unnamed protein product
Gene Aliases: AL024257; AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1; NM23-M1; NM23A; NME1
UniProt ID: (Human) P15531, (Mouse) P15532, (Rat) Q05982
Entrez Gene ID: (Human) 4830, (Mouse) 18102, (Rat) 191575
nucleotide binding
magnesium ion binding
DNA binding
RNA binding
endodeoxyribonuclease activity
deoxyribonuclease activity
nucleoside diphosphate kinase activity
protein histidine kinase activity
protein binding
ATP binding
3'-5'-exodeoxyribonuclease activity
kinase activity
transferase activity
phosphotransferase activity, phosphate group as acceptor
GDP binding
identical protein binding
ribosomal small subunit binding
ADP binding
metal ion binding
farnesyl diphosphate kinase activity
coenzyme A binding
succinyl-CoA binding
acetyl-CoA binding
RNA polymerase II regulatory region sequence-specific DNA binding
single-stranded DNA binding
GTP binding
intermediate filament binding
enzyme binding
protein kinase binding
gamma-tubulin binding
poly(A) RNA binding
kinase
GTP biosynthetic process
UTP biosynthetic process
CTP biosynthetic process
DNA catabolic process
apoptotic DNA fragmentation
isoprenoid metabolic process
endocytosis
nervous system development
lactation
negative regulation of cell proliferation
nucleotide metabolic process
nucleoside diphosphate metabolic process
nucleoside triphosphate biosynthetic process
cell differentiation
mammary gland development
protein hexamerization
regulation of fatty acid biosynthetic process
regulation of apoptotic process
ITP biosynthetic process
acetyl-CoA catabolic process
positive regulation of epithelial cell proliferation
negative regulation of myeloid leukocyte differentiation
purine nucleotide metabolic process
nucleoside diphosphate phosphorylation
pyrimidine nucleotide metabolic process
DNA metabolic process
negative regulation of gene expression
positive regulation of neuron projection development
phosphorylation
positive regulation of DNA binding
nucleic acid phosphodiester bond hydrolysis
integrin-mediated signaling pathway
response to amine
hippocampus development
response to testosterone
cellular response to drug
response to drug
negative regulation of apoptotic process
positive regulation of keratinocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
response to cAMP
cellular response to glucose stimulus
cellular response to fatty acid
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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