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Invitrogen
NME4 Polyclonal Antibody
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FIGURE: 1 / 1
NME4 Antibody (PA5-97017) in WB
Western blot analysis of NME4 in extracts of various cell lines. Samples were incubated with NME4 Polyclonal antibody (Product # PA5-97017) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-97017
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human NME4 (NP_0050001)
Conjugate
Form
Liquid
Concentration
0.43 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MGGLFWRSAL RGLRCGPRAP GPSLLVRHGS GGPSWTRERT LVAVKPDGVQ RRLVGDVIQR FERRGFTLVG MKMLQAPESV LAEHYQDLRR KPFYPALIRY MSSGPVVAMV WEGYNVVRAS RAMIGHTDSA EAAPGTIRGD FSVHISRNVI HASDSVEGAQ REIQLWFQSS ELVSWADGGQ HSSIHPA; Positive Samples: Jurkat, A-549, NCI-H460, BT-474, Mouse kidney, Mouse stomach, Rat brain; Cellular Location: Mitochondrion intermembrane space, Mitochondrion matrix, Peripheral membrane protein
Target Information
NME4 is a protein kinases, which are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: expressed in non-metastatic cells 4, protein; NDK; NDP kinase D; NDP kinase, mitochondrial; NDPKD; nm23-H4; nm23-M4; non-metastatic cells 4, protein expressed in; nucleoside diphosphate kinase 4; Nucleoside diphosphate kinase D; Nucleoside diphosphate kinase, mitochondrial
Gene Aliases: 2610027N22Rik; 2810024O08Rik; 5730493H09Rik; NDPK-D; nm23-H4; NM23-M4; NM23D; NM23H4; Nm23M4; NME4
UniProt ID: (Human) O00746, (Mouse) Q9WV84
Entrez Gene ID: (Human) 4833, (Rat) 685679, (Mouse) 56520
purine nucleotide metabolic process
nucleoside diphosphate phosphorylation
GTP biosynthetic process
pyrimidine nucleotide metabolic process
UTP biosynthetic process
CTP biosynthetic process
lipid transport
nucleoside metabolic process
nucleoside triphosphate biosynthetic process
nucleobase-containing small molecule interconversion
regulation of apoptotic process
nucleotide metabolic process
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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