Proteintech

NNMT Polyclonal Antibody

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NNMT Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 15123-1-AP (NNMT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NNMT Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15123-1-AP ( NNMT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

NNMT Antibody in Immunoprecipitation (IP) — IP result of anti-NNMT (IP:15123-1-AP, 4ug; Detection:15123-1-AP 1:300) with HepG2 cells lysate 2400ug.

Product Details

15123-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NNMT Fusion Protein Ag7197 (1-264 aa encoded by BC000234) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.21 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MESGFTSKD TYLSHFNPRD YLEKYYKFGS RHSAESQILK HLLKNLFKIF CLDGVKGDLL IDIGSGPTIY QLLSACESFK EIVVTDYSDQ NLQELEKWLK KEPEAFDWSP VVTYVCDLEG NRVKGPEKEE KLRQAVKQVL KCDVTQSQPL GAVPLPPADC VLSTLCLDAA CPDLPTYCRA LRNLGSLLKP GGFLVIMDAL KSSYYMIGEQ KFSSLPLGRE AVEAAVKEAG YTIEWFEVIS QSYSSTMANN EGLFSLVARK LSRPL (1-264 aa encoded by BC000234)

Target Information

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.