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Immunofluorescent analysis of NOLC1 showing staining in the cytoplasm and nucleolus of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a NOLC1 polyclonal antibody (Product # PA5-35938) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 442 and 668 of NOLC1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
140 kDa nucleolar phosphoprotein; HCV NS5A trans-regulated protein 13; HCV NS5A-transactivated protein 13; hepatitis C virus NS5A-transactivated protein 13; NOPP130; NOPP140; NS5ATP13; nucleolar 130 kDa protein; nucleolar and coiled-body phosphoprotein 1; nucleolar and coiled-body phosphprotein 1; nucleolar phosphoprotein p130; nucleolar protein p130
KIAA0035; NOLC1; NOPP130; NOPP140; NS5ATP13; P130