Immunohistochemical analysis of NOLC1 in paraffin-embedded human kidney following steamed antigen retrieval with a citrate buffer, pH 6, using a NOLC1 polyclonal antibody (Product# PA5-37912) at a dilution of 2ug/ml. Detection was performed using an HRP-conjugated secondary antibody.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-QVNSIKFDSE, from the internal region of NOLC1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat, dog and cow based on sequence homology.
Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.