Invitrogen

NOP14 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (11) NOP14 antibodies

Questions & Answers

Questions & Answers

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NOP14 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of NOP14 in human cell line U-2 OS shows positivity in nucleus & nucleoli. Samples were probed using a NOP14 Polyclonal Antibody (Product # PA5-58851).

NOP14 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of NOP14 in human hippocampus shows moderate nucleolar positivity in neurons. Samples were probed using a NOP14 Polyclonal Antibody (Product # PA5-58851).

NOP14 Antibody in Western Blot (WB) — Western blot analysis of NOP14 in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a NOP14 Polyclonal Antibody (Product # PA5-58851). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

NOP14 Antibody in Western Blot (WB) — Western blot analysis of NOP14 in mouse cell line NIH-3T3 and rat cell line NBT-II using a NOP14 Polyclonal Antibody (Product # PA5-58851).

NOP14 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-251MG cells were transfected with, control siRNA, NOP14 siRNA probe #1, and NOP14 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-NOP14 Polyclonal Antibody (Product # PA5-58851) used at a dilution of 0.4 µg/ml. Anti-GAPDH was used as a loading control. {KD}

Product Details

PA5-58851

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:500-1:1,000	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human NOP14 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2644739

Product Specific Information

Immunogen sequence: REKPKSRKEL IEELIAKSKQ EKRERQAQRE DALELTEKLD QDWKEIQTLL SHKTPKSENR DKKEKPKPDA YDMMVRELGF EMKAQPSNRM KT

Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 89%.

Target Information

Involved in nucleolar processing of pre-18S ribosomal RNA. Has a role in the nuclear export of 40S pre-ribosomal subunit to the cytoplasm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: NOP14 nucleolar protein homolog; Nucleolar complex protein 14; Nucleolar protein 14; probable nucleolar complex protein 14

Gene Aliases: 2610033H07Rik; C4orf9; NOL14; NOP14; RES4-25; RES425; RGD1305605; UTP2

UniProt ID: (Human) P78316, (Mouse) Q8R3N1

Entrez Gene ID: (Human) 8602, (Mouse) 75416, (Rat) 289724

Molecular Function: RNA metabolism protein nucleic acid metabolism protein

endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA processing maturation of SSU-rRNA ribosomal small subunit biogenesis biological_process ribosome biogenesis

Process(es)

enzyme binding snoRNA binding poly(A) RNA binding