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Bethyl Laboratories
NOSIP Polyclonal Antibody
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FIGURE: 1 / 1
NOSIP Antibody (A305-086A) in WB
Detection of human NOSIP by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NOSIP antibody (Product # A305-086A) (lot A305-086A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A305-086A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 100 to 150 of human Nitric oxide synthase-interacting protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1. 2, Nav1. 3, Nav1. 5, Nav1. 7, Nav1. 8, Kv1. 3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase NOSIP; eNOS-interacting protein; Nitric oxide synthase-interacting protein; RING-type E3 ubiquitin transferase NOSIP; unnamed protein product
Gene Aliases: CGI-25; NOSIP
UniProt ID: (Human) Q9Y314
Entrez Gene ID: (Human) 51070
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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