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Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Immunohistochemistry (Paraffin) (IHC (P)) |
1:200 | - |
Western Blot (WB) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide derived from C-terminus of human notch-1 |
Conjugate |
Unconjugated |
Form |
Liquid |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.6, with 1% BSA |
Contains |
0.1% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2549988 |
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is breast or ovarian carcinoma.
The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: major type A protein; Motch A; mT14; Neurogenic locus notch homolog protein 1; NEXT; NICD; Notch 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; Notch gene homolog 1; Notch homolog 1, translocation-associated; p300; RP23-306D20.12; RP23-306D20.12-001; Translocation-associated notch protein TAN-1; transmembrane receptor Notch1
Gene Aliases: 9930111A19Rik; AOS5; AOVD1; hN1; lin-12; Mis6; Motch; N1; NOTCH1; TAN1
UniProt ID: (Human) P46531, (Mouse) Q8K428
Entrez Gene ID: (Human) 4851, (Mouse) 18128
Molecular Function:
cell adhesion molecule
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