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Invitrogen

NOTCH2 (Cleaved Asp1733) Polyclonal Antibody

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Product Details

PA5-99443

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human NOTCH2(Accession Q04721), corresponding to amino acid residues L1717-S1741.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2818376

Product Specific Information

Antibody detects endogenous levels of fragment of activated Notch2 resulting from cleavage adjacent to Asp1733.

Target Information

NOTCH2 (Cleaved Asp1733) refers to the activated intracellular domain of the Notch2 receptor generated following gamma-secretase-mediated proteolytic cleavage at aspartic acid residue 1733. NOTCH2 is encoded by the NOTCH2 gene and functions as a single-pass transmembrane receptor in the conserved Notch signaling pathway, which regulates cell fate determination, differentiation, proliferation, and survival. Upon ligand engagement by Delta-like or Jagged family members on adjacent cells, NOTCH2 undergoes sequential proteolytic cleavages that culminate in intramembrane cleavage and release of the Notch2 intracellular domain (NICD2). The cleaved intracellular domain translocates to the nucleus, where it associates with the DNA-binding protein RBPJ and transcriptional co-activators to drive expression of target genes, including members of the HES and HEY families. Detection of NOTCH2 cleaved at Asp1733 is widely used as a marker of active Notch2 signaling in developmental biology, stem cell regulation, immunology, and cancer research.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: membrane bound receptor; Motch B; Neurogenic locus notch homolog protein 2; Notch 2; Notch gene homolog 2; Notch homolog 2; putative cell-fate determining protein; transmembrane protein; unnamed protein product

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Gene Aliases: AGS2; AI853703; HJCYS; hN2; N2; NOTCH2

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UniProt ID: (Human) Q04721, (Mouse) O35516

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Entrez Gene ID: (Human) 4853, (Mouse) 18129, (Rat) 29492

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Function(s)
transcription coactivator activity calcium ion binding protein binding enzyme binding signaling receptor activity NF-kappaB binding receptor activity cell adhesion molecule
Process(es)
negative regulation of transcription from RNA polymerase II promoter in utero embryonic development cell fate determination liver development placenta development heart looping morphogenesis of an epithelial sheet marginal zone B cell differentiation inflammatory response to antigenic stimulus atrioventricular node development pulmonary valve morphogenesis regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter apoptotic process humoral immune response Notch signaling pathway determination of left/right symmetry nervous system development axon guidance cell proliferation positive regulation of cell proliferation organ morphogenesis negative regulation of macromolecule biosynthetic process negative regulation of gene expression positive regulation of keratinocyte proliferation hemopoiesis cell differentiation embryonic limb morphogenesis BMP signaling pathway positive regulation of BMP signaling pathway multicellular organism growth intracellular signal transduction intrahepatic bile duct development wound healing defense response to bacterium regulation of apoptotic process myeloid dendritic cell differentiation positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of Ras protein signal transduction bone remodeling regulation of developmental process positive regulation of smooth muscle cell differentiation cardiac septum morphogenesis atrial septum morphogenesis placenta blood vessel development ciliary body morphogenesis positive regulation of ERK1 and ERK2 cascade left/right axis specification cellular response to tumor cell proximal tubule development glomerular visceral epithelial cell development glomerular capillary formation hepatocyte proliferation liver morphogenesis positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of hemopoiesis cholangiocyte proliferation regulation of osteoclast development transcription, DNA-templated cell cycle arrest multicellular organism development negative regulation of cell proliferation cell growth interleukin-4 secretion tissue regeneration
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