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Invitrogen
NOTCH3 Polyclonal Antibody
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FIGURE: 1 / 2
NOTCH3 Antibody (PA5-144676) in WB
Western blot analysis of NOTCH3 in different various lysates. Lane 1: Hela cell lysate; Lane 2: MCF-7 cell lysate; Lane 3: PANC-1 cell lysate; Lane 4: HUVEC cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 244 kDa. Observed band size: 90 kDa. Exposure time: 1 minute 30 seconds; 6% SDS-PAGE gel. Primary antibody NOTCH3 polyclonal antibody (Product # PA5-144676) with a dilution of 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1:300,000 was used... View More
Product Details
PA5-144676
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human NOTCH3 aa 2022-2321.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Amount
200 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: Hela cell lysate, MCF-7 cell lysate, PANC-1 cell lysate, HUVEC cell lysate, Hela.
Predicted band size: 244 kDa
Subcellular Location: Cell membrane; Nucleus.
Target Information
NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction (with its cell-bound ligands delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human. NOTCH3 functions as a receptor for membrane-bound ligands Jagged1, Jagged2, and Delta1 to regulate cell-fate determination. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Neurogenic locus notch homolog protein 3; Notch 3; Notch homolog 3
Gene Aliases: CADASIL; CADASIL1; CASIL; IMF2; LMNS; NOTCH3
UniProt ID: (Human) Q9UM47
Entrez Gene ID: (Human) 4854
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
Notch signaling pathway
axon guidance
neuroblast differentiation
cell differentiation
neuron differentiation
forebrain development
negative regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of smooth muscle cell proliferation
neuron fate commitment
artery morphogenesis
regulation of developmental process
glomerular capillary formation
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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