Invitrogen

NOV Polyclonal Antibody

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NOV Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of CCN3 in paraformaldehyde-fixed HeLa cells using a CCN3 polyclonal antibody (Product # PA5-27893) at a 1:200 dilution.

NOV Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CCN3 in paraffin-embedded gastric carcinoma N87 xenograft using a CCN3 polyclonal antibody (Product # PA5-27893) at a dilution of 1:100.

NOV Antibody in Western Blot (WB) — Western Blot analysis of NOV was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a NOV Polyclonal Antibody (Product # PA5-27893) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:10,000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 57 and 357 of Human CCN3

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2545369

Product Specific Information

Recommended positive controls: 293T, mouse brain, human CCN3-transfected 293T cells.

Predicted reactivity: Mouse (84%), Rat (83%), Rhesus Monkey (100%), Bovine (82%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CCN family member 3; Cellular communication network factor 3; IBP-9; IGF-binding protein 9; Insulin-like growth factor-binding protein 9; Nephro blastoma-overexpressed gene protein homolog; nephroblastoma overexpressed gene protein homolog; Nephroblastoma-overexpressed gene protein homolog; NovH; Protein NOV homolog

Gene Aliases: C130088N23Rik; CCN3; IBP-9; IGFBP-9; IGFBP9; NOV; NOVH

UniProt ID: (Human) P48745, (Mouse) Q64299

Entrez Gene ID: (Human) 4856, (Mouse) 18133

Molecular Function: growth factor signaling molecule