Invitrogen

NOV Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (13) NOV antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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NOV Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of NOV in human cell line U-2 OS shows positivity in vesicles. Samples were probed using a NOV Polyclonal Antibody (Product # PA5-54055).

NOV Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of NOV in human adrenal gland using NOV Polyclonal Antibody (Product # PA5-54055) shows moderate to strong cytoplasmic positivity in glandular cells.

NOV Antibody in Western Blot (WB) — Western blot analysis of NOV in control (vector only transfected HEK293T lysate) and NOV over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a NOV Polyclonal Antibody (Product # PA5-54055).

NOV Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of NOV demonstrates antibody specificity. Immunohistochemical analysis of NOV using anti-NOV Polyclonal Antibody (Product # PA5-54055), shows significant staining of NOV in human adrenal gland and shows minimal or weak staining in human human skeletal muscle tissues. The relative expression levels of NOV within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-54055

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:200-1:500	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human NOV View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.3 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2644755

Product Specific Information

Immunogen sequence: SCCLVCARQR GESCSDLEPC DESSGLYCDR SADPSNQTGI CTAVEGDNCV FDGVIYRSGE KFQPSCKFQC TCRDGQIGC

Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 80%.

Target Information

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CCN family member 3; Cellular communication network factor 3; IBP-9; IGF-binding protein 9; Insulin-like growth factor-binding protein 9; Nephro blastoma-overexpressed gene protein homolog; nephroblastoma-overexpressed gene protein homolog; NovH; Protein NOV homolog

Gene Aliases: CCN3; IBP-9; IGFBP-9; IGFBP9; NOV; NOVH

UniProt ID: (Human) P48745

Entrez Gene ID: (Human) 4856

Molecular Function: growth factor