Search
Invitrogen
NOX1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 2
NOX1 Antibody (PA5-88280) in IHC (P)
Immunohistochemistry analysis of NOX1 in paraffin-embedded human colon carcinoma. Samples were incubated with NOX1 Polyclonal antibody (Product # PA5-88280) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-88280
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 418-564 of human NOX1 (NP_0089832?)
Conjugate
Form
Liquid
Concentration
0.98 mg/mL
Amount
98 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: WYKFQCADHN LKTKKIYFYW ICRETGAFSW FNNLLTSLEQ EMEELGKVGF LNYRLFLTGW DSNIVGHAAL NFDKATDIVT GLKQKTSFGR PMWDNEFSTI ATSHPKSVVG VFLCGPRTLA KSLRKCCHRY SSLDPRKVQF YFNKENF
Positive Samples: Rat liver; Cellular Location: Cell projection, Multi-pass membrane protein, invadopodium membrane
Target Information
Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv. NOH-1S is a voltage-gated proton channel that participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H (+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. NOX1 have the potential to be effective treatments for a range of ischemic diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: GP91 phox homolog; mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating); Mitogenic oxidase 1; MOX-1; Mox1; similar to gp91phox; NADH/NADPH mitogenic oxidase subunit P65-MOX; NADPH oxidase 1; NADPH oxidase 1 alpha; NADPH oxidase homolog-1; NOH-1; NOH-1L; NOH-1Lv; NOH-1S; NOX-1; NOX1; unnamed protein product
Gene Aliases: GP91-2; MOX1; NOH-1; NOH-1L; NOH1; Nox-1; NOX1; NOX1a; NOX1alpha
UniProt ID: (Human) Q9Y5S8, (Mouse) Q8CIZ9
Entrez Gene ID: (Human) 27035, (Mouse) 237038
protein binding
NAD(P)H oxidase activity
superoxide-generating NADPH oxidase activity
oxidoreductase activity
small GTPase binding
metal ion binding
NADP binding
oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
superoxide-generating NADPH oxidase activity
N-ethylmaleimide reductase activity
voltage-gated proton channel activity
reduced coenzyme F420 dehydrogenase activity
sulfur oxygenase reductase activity
malolactic enzyme activity
NADPH:sulfur oxidoreductase activity
Rac GTPase binding
epoxyqueuosine reductase activity
oxidase
MAPK cascade
angiogenesis
regulation of systemic arterial blood pressure by renin-angiotensin
NADP metabolic process
defense response
inflammatory response
signal transduction
JNK cascade
regulation of blood pressure
positive regulation of cell proliferation
intrinsic apoptotic signaling pathway in response to oxidative stress
positive regulation of vascular endothelial growth factor production
cell migration
extracellular matrix organization
superoxide anion generation
hydrogen peroxide metabolic process
positive regulation of MAPK cascade
positive regulation of integrin biosynthetic process
respiratory burst
positive regulation of JNK cascade
positive regulation of smooth muscle cell proliferation
cellular response to hyperoxia
oxygen metabolic process
positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
response to reactive oxygen species
superoxide metabolic process
transport
proton transport
regulation of ion transmembrane transport
intracellular pH elevation
oxidation-reduction process
cation transmembrane transport
cellular stress response to acidic pH
positive regulation of reactive oxygen species metabolic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support