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Invitrogen
NOX3 Polyclonal Antibody
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FIGURE: 1 / 5
NOX3 Antibody (PA5-38036) in ICC/IF
Immunofluorescent analysis of Nox3 (Violet) in mouse bone marrow cells (Up). The cells were fixed with 2% paraformaldehyde in PBS for 30 minutes at RT, permeabilized with 0.5% Triton X-100 in PBS for 30 minutes at RT, and blocked with blocking buffer for 4 hours in cold room. Cells were stained with rabbit polyclonal anti-Nox3 antibody (Product # PA5-38036) at a dilution of 1:200 in blocking buffer overnight in cold room, wash 5 times with 1 xPBS, incubated with goat anti-rabbit IgG antibody conjugated with Cy5 (Product # A10523) at a dilu... View More
Product Details
PA5-38036
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
15 amino acid peptide near the amino terminus of human NOX3
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA5-38036 detects Nox3 in mouse bone marrow and humanG-CSF mobilized CD34+ cells in immunofluorescence analysis.
A recommended positive control is 293 cell lysate.
NOX3 is not expected to cross-react with other NOX proteins.
Target Information
The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide. NOX3 is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith. It has been suggested that NOX3 is activated by the Transient Receptor Potential Vanilloid 1 (TRPV1), and this activity causes increased levels of reactive oxygen species in the inner ear, which in turn leads to STAT1-mediated inflammation and hearing loss.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GP91-3; gp91phox homolog 3; head-tilt; Mitogenic oxidase 2; MOX-2; NADPH oxidase 3; NADPH oxidase catalytic subunit-like 3; NAPDH oxidase 3; NOX-3
Gene Aliases: GP91-3; het; MOX-2; MOX2; nmf250; NOX3
UniProt ID: (Human) Q9HBY0, (Mouse) Q672J9
Entrez Gene ID: (Human) 50508, (Mouse) 224480
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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