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Invitrogen
NOX4 Polyclonal Antibody
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FIGURE: 1 / 5
NOX4 Antibody (PA5-95083) in IHC (P)
Immunohistochemistry analysis of NOX4 in paraffin-embedded rat kidney tissues. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with NOX4 polyclonal antibody (Product # PA5-95083) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.
Product Details
PA5-95083
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse NADPH oxidase 4 (561-578aa NRNNSYGTKFEYNKES).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide, 0.05mg thimerosal
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human SW620 whole cell, human HK-2 whole cell, human HL-60 whole cell, human 293T whole cell, human SW579 whole cell, human SK-OV-3 whole cell, rat kidney tissue. IHC: rat kidney tissue. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Oxygen sensing is essential for homeostasis in all aerobic organisms. A phagocyte-type oxidase, similar to that responsible for the production of large amounts of reactive oxygen species (ROS) in neutrophil granulocytes, with resultant antimicrobial activity, has been postulated to function in the kidney as an oxygen sensor that regulates the synthesis of erythropoietin in the renal cortex. NOX4 has a role as a redox messenger in the activation of intracellular signaling pathways leading (or contributing) to mitochondriogenesis, cell survival, and differentiation in hematopoietic stem cells. Data suggests that NOX4 provides a novel link between the insulin receptor and the generation of cellular reactive oxygen species that enhances insulin signal transduction.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX-1; KOX1; NADPH oxidase 4; NOX 4; NOX-4; Renal NAD(P)H-oxidase; Superoxide-generating NADPH oxidase 4
Gene Aliases: AI648021; KOX; KOX-1; NOX4; RENOX
UniProt ID: (Human) Q9NPH5, (Rat) Q924V1, (Mouse) Q9JHI8
Entrez Gene ID: (Human) 50507, (Rat) 85431, (Mouse) 50490
nucleotide binding
protein binding
electron carrier activity
NAD(P)H oxidase activity
superoxide-generating NADPH oxidase activity
oxygen sensor activity
heme binding
flavin adenine dinucleotide binding
modified amino acid binding
oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
oxidoreductase activity
oxidase
response to reactive oxygen species
cell morphogenesis
response to hypoxia
superoxide metabolic process
inflammatory response
cell aging
negative regulation of cell proliferation
gene expression
positive regulation of smooth muscle cell migration
superoxide anion generation
positive regulation of apoptotic process
positive regulation of MAP kinase activity
bone resorption
homocysteine metabolic process
positive regulation of stress fiber assembly
positive regulation of protein kinase B signaling
cardiac muscle cell differentiation
oxidation-reduction process
positive regulation of ERK1 and ERK2 cascade
cellular response to cAMP
cellular response to glucose stimulus
cellular response to gamma radiation
cellular response to transforming growth factor beta stimulus
reactive oxygen species metabolic process
positive regulation of reactive oxygen species metabolic process
positive regulation of DNA biosynthetic process
aging
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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