|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptides corresponding to residues 51-66 and 75-90 of mouse Noxa.|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: human, mouse or rat thymus tissue lysate or protein, antigen standard for PMAIP1 (transient overexpression lysate).
Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage) (1). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103-amino acid protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al (1) have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.