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Invitrogen
NPAS2 Polyclonal Antibody
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FIGURE: 1 / 1
NPAS2 Antibody (PA5-95462) in WB
Western blot analysis of NPAS2 in Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate, Lane 3: human COLO-320 whole cell lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with NPAS2 polyclonal antibody (Product # PA5-95462) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and develo... View More
Product Details
PA5-95462
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human NPAS2 recombinant protein (Position: D474-Q578).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Human NPAS2 shares 88.1% amino acid (aa) sequence identity with mouse NPAS2.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat brain tissue, mouse brain tissue, human COLO-320 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Members of the basic helix-loop-helix-PER-ARNT-SIM (bHLH-PAS) family are transcription factors that contain a bHLH DNA binding domain located amino-terminal to a PAS domain. Neuronal PAS domain protein 2 (NPAS2, also designated PAS 4/MOP4) is a member of the bHLH-PAS family and the PAS superfamily. NPAS2, which maps to chromosome 2p11.2-2q13, is expressed primarily in the neurons during the first week of postnatal development. The pattern of NPAS2 expression temporally matches the development of learning and memory, and spatially matches the frontal association/limbic forebrain pathway. NPAS2 may serve a regulatory role in the development and maintenance of long-term memory, and may be required for the processing of complex sensory information. NPAS2 and MOP3 form a transcriptionally active heterodimer which binds to a CACGTGA-containing DNA element and drives transcription from a linked luciferase reporter gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: a member of the bHLH-PAS family.; Basic-helix-loop-helix-PAS protein MOP4; bHLHe9; Class E basic helix-loop-helix protein 9; Member of PAS protein 4; member of PAS superfamily 4; Neuronal PAS domain-containing protein 2; Neuronal PAS2; PAS domain-containing protein 4; unnamed protein product
Gene Aliases: BHLHE9; MOP4; NPAS2; PASD4
UniProt ID: (Human) Q99743, (Mouse) P97460
Entrez Gene ID: (Human) 4862, (Mouse) 18143, (Rat) 316351
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
metal ion binding
protein dimerization activity
Hsp90 protein binding
sequence-specific double-stranded DNA binding
signal transducer activity
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cellular response to DNA damage stimulus
central nervous system development
response to xenobiotic stimulus
regulation of gene expression
circadian regulation of gene expression
positive regulation of DNA repair
positive regulation of transcription, DNA-templated
rhythmic process
response to redox state
positive regulation of behavioral fear response
transcription, DNA-templated
signal transduction
circadian sleep/wake cycle
regulation of circadian rhythm
locomotor rhythm
positive regulation of transcription from RNA polymerase II promoter
negative regulation of cell death
regulation of response to DNA damage stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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