Proteintech

NPHP3 Polyclonal Antibody

NPHP3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 11

NPHP3 Antibody (22026-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 22026-1-AP (NPHP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

NPHP3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

NPHP3 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

22026-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 5 publications 5 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

NPHP3 Fusion Protein Ag16888 (1-131 aa encoded by BC068082)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.51 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MGTASSLVS PAGGEVIEDT YGAGGGEACE IPVEVKPKAR LLRNSFRRGA GAAAGAGPGS LPRGVGAGGL LGASFKSTGS SVPELEYAAA EYERLRKEYE IFRVSKNQEL LSMGRREAKL DTENKRLRAE LQ (1-131 aa encoded by BC068082)

Target Information

Required for normal ciliary development and function. Inhibits disheveled-1-induced canonical Wnt-signaling activity and may also play a role in the control of non-canonical Wnt signaling which regulates planar cell polarity. Probably acts as a molecular switch between different Wnt signaling pathways. Required for proper convergent extension cell movements.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Single-molecule imaging in the primary cilium.

Methods in cell biology

Weiss LE,Love JF,Yoon J,Comerci CJ,Milenkovic L,Kanie T,Jackson PK,Stearns T,Gustavsson AK

Fri May 12 00:00:00 UTC 2023

Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to cilia.

Molecular biology of the cell

Palicharla VR,Hwang SH,Somatilaka BN,Legué E,Shimada IS,Familiari NE,Tran VM,Woodruff JB,Liem KF,Mukhopadhyay S

Wed Mar 01 00:00:00 UTC 2023

Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses.

International journal of molecular sciences

Suiwal S,Dembla M,Schwarz K,Katiyar R,Jung M,Carius Y,Maxeiner S,Lauterbach MA,Lancaster CRD,Schmitz F

Mon Jun 27 00:00:00 UTC 2022

ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition.

American journal of human genetics

Alkanderi S,Molinari E,Shaheen R,Elmaghloob Y,Stephen LA,Sammut V,Ramsbottom SA,Srivastava S,Cairns G,Edwards N,Rice SJ,Ewida N,Alhashem A,White K,Miles CG,Steel DH,Alkuraya FS,Ismail S,Sayer JA

Thu Oct 04 00:00:00 UTC 2018

ARL3 Mutations Cause Joubert Syndrome by Disrupting Ciliary Protein Composition.

American journal of human genetics

Alkanderi S,Molinari E,Shaheen R,Elmaghloob Y,Stephen LA,Sammut V,Ramsbottom SA,Srivastava S,Cairns G,Edwards N,Rice SJ,Ewida N,Alhashem A,White K,Miles CG,Steel DH,Alkuraya FS,Ismail S,Sayer JA

Thu Oct 04 00:00:00 UTC 2018

Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelium cells.

Human molecular genetics

Schwarz N,Carr AJ,Lane A,Moeller F,Chen LL,Aguilà M,Nommiste B,Muthiah MN,Kanuga N,Wolfrum U,Nagel-Wolfrum K,da Cruz L,Coffey PJ,Cheetham ME,Hardcastle AJ

Sun Feb 15 00:00:00 UTC 2015

Translational read-through of the RP2 Arg120stop mutation in patient iPSC-derived retinal pigment epithelium cells.

Human molecular genetics

Schwarz N,Carr AJ,Lane A,Moeller F,Chen LL,Aguilà M,Nommiste B,Muthiah MN,Kanuga N,Wolfrum U,Nagel-Wolfrum K,da Cruz L,Coffey PJ,Cheetham ME,Hardcastle AJ

Sun Feb 15 00:00:00 UTC 2015

Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.

Nature methods

Su S,Phua SC,DeRose R,Chiba S,Narita K,Kalugin PN,Katada T,Kontani K,Takeda S,Inoue T

Fri Nov 01 00:00:00 UTC 2013

Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia.

Nature methods

Su S,Phua SC,DeRose R,Chiba S,Narita K,Kalugin PN,Katada T,Kontani K,Takeda S,Inoue T

Fri Nov 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: cilia and flagella associated protein 31; Meckel syndrome, type 7; Nephrocystin-3; nephronophthisis 3 (adolescent)

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Gene Aliases: 3632410F03Rik; AI550417; C230078J01; CFAP31; D330020E01Rik; KIAA2000; MKS7; NPH3; NPHP3; pcy; RGD1563856; RHPD; RHPD1; SLSN3

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UniProt ID: (Human) Q7Z494, (Mouse) Q7TNH6

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Entrez Gene ID: (Dog) 100856544, (Human) 27031, (Mouse) 74025, (Rat) 363126

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Function(s)

protein binding molecular_function

Process(es)

kidney development heart looping atrial septum development lipid metabolic process establishment or maintenance of cell polarity determination of left/right symmetry Wnt signaling pathway extracellular matrix organization lung development regulation of cAMP metabolic process determination of pancreatic left/right asymmetry photoreceptor cell maintenance maintenance of organ identity convergent extension involved in gastrulation cilium morphogenesis kidney morphogenesis determination of intestine left/right asymmetry determination of stomach left/right asymmetry determination of liver left/right asymmetry ureter development negative regulation of canonical Wnt signaling pathway epithelial cilium movement involved in determination of left/right asymmetry regulation of Wnt signaling pathway, planar cell polarity pathway regulation of planar cell polarity pathway involved in neural tube closure

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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NPHP3 Polyclonal Antibody

NPHP3 Antibody (22026-1-AP) in ICC/IF

Product Details

22026-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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