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Invitrogen
NRF1 Polyclonal Antibody
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FIGURE: 1 / 21
NRF1 Antibody (PA5-95573) in IHC (P)
Immunohistochemistry analysis of NRF1 in paraffin-embedded human gastric cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with NRF1 polyclonal antibody (Product # PA5-95573) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex a... View More
Product Details
PA5-95573
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human NRF1 recombinant protein (Position: D246-Q503).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human MDA-MB-231 whole cell. IHC: human placenta tissue, human ovary cancer tissue, mouse intestine tissue, mouse kidney tissue, mouse spleen tissue, rat heart tissue, rat kidney tissue, human appendicitis tissue, human gastric cancer tissue, human tonsil tissue, mouse cardiac muscle tissue, mouse liver tissue, rat brain tissue, rat intestine tissue, rat liver tissue, rat lung tissue, rat skeletal muscle tissue, rat spleen tissue. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Alpha palindromic-binding protein; Alpha-pal; EWG; NRF-1; Nuclear respiratory factor 1; nuclear respiratory factor-1; unnamed protein product
Gene Aliases: ALPHA-PAL; C87038; D6Ertd415e; Nfe2l1_retired; NRF1
UniProt ID: (Human) Q16656, (Mouse) Q9WU00
Entrez Gene ID: (Human) 4899, (Rat) 312195, (Mouse) 18181
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein homodimerization activity
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
molecular_function
DNA-binding transcription factor
regulation of transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
response to hypoxia
transcription, DNA-templated
mitochondrion organization
organ regeneration
response to estradiol
response to lipopolysaccharide
response to folic acid
response to electrical stimulus
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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